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Highly Selective Differential Pulse Voltammetric Determination of Uric Acid Using Modified Glassy Carbon Electrode Publisher



Movlaee K1 ; Norouzi P1, 2 ; Beitollahi H3 ; Rezapour M4 ; Larijani B5
Authors
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Authors Affiliations
  1. 1. Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran
  2. 2. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
  4. 4. IP Department, Research Institute of the Petroleum Industry (RIPI), P.O. Box 14665-137, Tehran, Iran
  5. 5. Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Electrochemical Science Published:2017


Abstract

A nanocomposite was prepared by incorporating Fe3O4 onto graphene sheets, and then a layer of SiO2 were deposited on the surface of the Fe3O4/graphene composites. This modified electrode was used for electrochemical determination of uric acid (UA). Cyclic voltammetry, differential pulse voltammetry and chronoamperometry were used to investigate the electrochemical behavior of uric acid at the chemically modified electrode. According to the results, Fe3O4@SiO2/GO/GCE showed high electrocatalytic activity for uric acid oxidation, producing a sharp oxidation peak current at 330 mV vs. Ag/AgCl reference electrode at pH 7.0. The peak current was linearly dependent on uric acid concentration over the range of 0.5 to 250.0 μM with the detection limit (3σ) of 0.07 μM. The proposed method was successfully applied as a rapid, highly selective, simple, and precise one to determine uric acid in urine. © 2017 The Authors.