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Sensitive Electrochemical Determination of Rifampicin Using Gold Nanoparticles/Poly-Melamine Nanocomposite Publisher



Amidi S1 ; Ardakani YH2 ; Amiriaref M2 ; Ranjbari E2 ; Sepehri Z3 ; Bagheri H4
Authors
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Authors Affiliations
  1. 1. Department of Medicinal Chemistry, School of Pharmacy, Protein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Biopharmaceutics and Pharmacokinetics Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran
  3. 3. Department of Internal Medicine, Zabol University of Medical Sciences, Zabol, Iran
  4. 4. Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: RSC Advances Published:2017


Abstract

In this work, a novel electrochemical sensing platform was successfully prepared by electrodeposition of gold nanoparticles on the electropolymerized layer of poly-melamine modified pre-anodized glassy carbon electrode. The surface morphology and electrochemical behaviour of the modified electrode (PMel-Aunano/GCE∗) were characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The proposed sensor remarkably increased the oxidation responses of rifampicin (RIF) associated with a significant decrease in its peak over-potentials of about 0.10 V based on the integration of the beneficial effects of a conductive PMel (with excellent electrocatalytic activity) and gold nanoparticles (with prominent electrochemical properties and large surface area), which exhibits the properties of their individual components with synergistic effects. The results demonstrated that voltammetric determination of RIF achieved favourably and the modified electrode represents two oxidation peaks for rifampicin at potentials approximately 0.09 V (peak 1) and 0.70 V (peak 2) in phosphate buffer solution with a linear range of 0.08-15.00 μM and a detection limit of 0.03 μM. The developed sensor is stable, reproducible and feasible for long-term use in biological samples with satisfactory results for RIF detection. © 2017 The Royal Society of Chemistry.