Anti-Differentiation Non-Coding Rna, Ancr, Is Differentially Expressed in Different Types of Brain Tumors Publisher Pubmed



Malakootian M^{1, 2} ; Mirzadeh Azad F¹ ; Fouani Y¹ ; Taheri Bajgan E¹ ; Saberi H^{3, 4} ; Mowla SJ¹ Show Affiliations
Source: Journal of Neuro-Oncology Published:2018

Abstract

Long non-coding RNAs (lncRNAs) are important modulators of various cellular and molecular events, including cancer-associated pathways. The Anti-differentiation ncRNA (ANCR) is a key regulator of keratinocyte differentiation, where its expression is necessary to maintain epidermal progenitor’s cells. Herein, we investigated the expression pattern of ANCR in the course of neural differentiation. Moreover, we used published RNAseq data and clinical samples to evaluate the alteration of ANCR expression in different cell types and brain tumors. Furthermore, we manipulated ANCR expression in glioma cell lines to clarify a potential functional role for ANCR in tumorigenesis. Our qRT-PCR results revealed a significant upregulation of ANCR in more malignant and less differentiated types of brain tumors (P = 0.03). This data was in accordance with down regulation of ANCR during neural differentiation. ANCR suppression caused an elevation in apoptosis rate, as well as a G1 cell cycle arrest in glioblastoma cell line. Altogether, our data demonstrated that ANCR may play a role in glioma genesis and that it could be considered as a potential diagnostic and therapeutic target to combat brain cancers. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.