Nuclear Magnetic Resonance -Based Metabolomics Analysis of Patients Exposed to Sulfur Mustard in Different Stages Using Random Forest Method



Nobakht M Gh BF^{1, 3} ; Aliannejad R² ; Oskouie AA³ ; Fathi F⁴ ; Sahakhah HA⁵ ; Rezaeitavirani M¹ Show Affiliations
Source: Koomesh Published:2016

Abstract

Introduction: Metabolomics is a powerful technique for determination of biomarkers. Here, we aimed to determine discriminatory metabolomic profiles in different stages of sulfur mustard-exposed patients (SMEPs). Materials and methods: Nuclear magnetic resonance spectroscopy was used to analyze serum samples from 17 SMEPs (normal group patients) and 17 SMEPs (severe group patients). Multivariate statistical analysis using random forest (RF) was performed on a ‘training set’ (70% of the total sample) in order to produce a discriminatory model classifying two groups of patients, and the model tested in the remaining subjects. Results: A classification model was derived using data from the training set with an area under the receiver operating curve (AUC) of 0.87. In the test set (the remaining 30% of subjects), the AUC was 0.8, thus RF model had good predictive power. We observed significant changes in lipid, amino acids and energy metabolism between two groups of patients. Conclusion: Nuclear magnetic resonance spectroscopy of serum successfully differentiates two groups of patients exposed to sulfur mustard. This technique has the potential to provide novel diagnostics and identify novel pathophysiological mechanisms, biomarkers and therapeutic targets. © 2016, Semnan University of Medical Sciences. All rights reserved.