Neuroprotective Effect of Propolis Polyphenol-Based Nanosheets in Cellular and Animal Models of Rotenone-Induced Parkinson’S Disease Publisher Pubmed



Mamashli F¹ ; Meratan AA² ; Ghasemi A¹ ; Obeidi N³ ; Salmani B² ; Atarod D¹ ; Pirhaghi M¹ ; Moosavimovahedi F¹ ; Mohammadzaheri M¹ ; Shahsavani MB⁴ ; Habibikelishomi Z¹ ; Goliaei B¹ ; Gholami M⁵ ; Saboury AA¹ Show Affiliations
Source: ACS Chemical Neuroscience Published:2023

Abstract

Considering the central role of oxidative stress in the onset and progress of Parkinson’s diseases (PD), search for compounds with antioxidant properties has attracted a growing body of attention. Here, we compare the neuroprotective effect of bulk and nano forms of the polyphenolic fraction of propolis (PFP) against rotenone-induced cellular and animal models of PD. Mass spectrometric analysis of PFP confirmed the presence of multiple polyphenols including kaempferol, naringenin, coumaric acid, vanillic acid, and ferulic acid. In vitro cellular experiments indicate the improved efficiency of the nano form, compared to the bulk form, of PFP in attenuating rotenone-induced cytotoxicity characterized by a decrease in cell viability, release of lactate dehydrogenase, increased ROS generation, depolarization of the mitochondrial membrane, decreased antioxidant enzyme activity, and apoptosis induction. In vivo experiments revealed that while no significant neuroprotection was observed relating to the bulk form, PFP nanosheets were very effective in protecting animals, as evidenced by the improved behavioral and neurochemical parameters, including decreased lipid peroxidation, increased GSH content, and antioxidant enzyme activity enhancement. We suggest that improved neuroprotective effects of PFP nanosheets may be attributed to their increased water solubility and enrichment with oxygen-containing functional groups (such as OH and COOH), leading to increased antioxidant activity of these compounds. © 2023 American Chemical Society.