Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis

Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis Publisher Pubmed

Bayat M¹ ; Shojaei S² ; Bahrami N^{1, 3} ; Mohamadnia A^{4, 5} ; Shojaei P⁶ ; Bahrami N^{1, 3}

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1. Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Gandhi Hospital, Cell Therapy Center, Tehran, Iran
3. Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran
4. Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. Department of Chemistry, Shahid Chamran University, Ahvaz, Iran
7. Psychiatry, University of California, San Diego, United States

Source: Journal of Biomedical Materials Research - Part A Published:2017

Abstract

The aim of the present study was to assess the recombinant bonemorphogenetic protein 2 (RHBMP-2) with higher substantively and solubility for use in calcium phosphate scaffolds for better release in differentiation of mesenchymal stem cells to osteoblast cells. Using bioinformatics tools, two mutations (p. L10D and p. S12E) were chosen and applied in BMP2 CDS sequence to increase interaction with calcium derived composite. The new recombinant mutated sequence (BMP2mut) was synthesized and then subcloned to expression vector pBV220. Experimental data regarded functional protein expression in E. coli. Since no modification was made in the active sites of proteins namely β-sheets and α-helixes, not only was there any change in the specific activity occurred in the specific activity of the enzyme in comparison to its commercial counterpart, but also mesenchymal osteogenesis occurred more efficient on biphasic CaP scaffold model. As we hypothesized, use of negatively charged amino acids such as aspartate and glutamate in protein loops increased the interactions of BMP2-Ca2+ and resulted in its slower and more sustained released from CaP scaffolds compare to commercial RHBMP2. Our data suggested that new BMP2mut have greater osteoinductive capacity than RHBMP2 in the same time and dose than RHBMP2. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2799–2805, 2017. © 2017 Wiley Periodicals, Inc.

Style	Citing Format
MLA	Bayat M, et al.. "Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis." Journal of Biomedical Materials Research - Part A, vol. 105, no. 10, 2017, pp. 2799-2805.
APA	Bayat M, Shojaei S, Bahrami N, Mohamadnia A, Shojaei P, Bahrami N (2017). Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis. Journal of Biomedical Materials Research - Part A, 105(10), 2799-2805.
Chicago	Bayat M, Shojaei S, Bahrami N, Mohamadnia A, Shojaei P, Bahrami N. "Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis." Journal of Biomedical Materials Research - Part A 105, no. 10 (2017): 2799-2805.
Harvard	Bayat M et al. (2017) 'Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis', Journal of Biomedical Materials Research - Part A, 105(10), pp. 2799-2805.
Vancouver	Bayat M, Shojaei S, Bahrami N, Mohamadnia A, Shojaei P, Bahrami N. Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis. Journal of Biomedical Materials Research - Part A. 2017;105(10):2799-2805.
BibTex	@article{ author = {Bayat M and Shojaei S and Bahrami N and Mohamadnia A and Shojaei P and Bahrami N}, title = {Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis}, journal = {Journal of Biomedical Materials Research - Part A}, volume = {105}, number = {10}, pages = {2799-2805}, year = {2017} }
RIS	TY - JOUR AU - Bayat M AU - Shojaei S AU - Bahrami N AU - Mohamadnia A AU - Shojaei P AU - Bahrami N TI - Protein Engineering of Recombinant Human Bone Morphogenetic Protein 2 With Higher Interaction With Ca Phosphate Based Scaffold Used for Osteogenesis JO - Journal of Biomedical Materials Research - Part A VL - 105 IS - 10 SP - 2799 EP - 2805 PY - 2017 ER -

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