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Egg Shell-Derived Calcium Phosphate/Carbon Dot Nanofibrous Scaffolds for Bone Tissue Engineering: Fabrication and Characterization Publisher Pubmed



Shafiei S1 ; Omidi M2 ; Nasehi F3 ; Golzar H4 ; Mohammadrezaei D5 ; Rezai Rad M3, 6 ; Khojasteh A3, 6
Authors
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Authors Affiliations
  1. 1. Oral and maxillofacial surgery resident, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Marquette University School of Dentistry, Milwaukee, WI, United States
  3. 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Chemistry & Waterloo Institute for Nanotechnology (WIN), University of Waterloo, Waterloo, Ontario, Canada
  5. 5. Department of Applied Mathematics, University of Waterloo, Waterloo, Canada
  6. 6. Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Materials Science and Engineering C Published:2019


Abstract

Recent exciting findings of the particular properties of Carbon dot (CDs) have shed light on potential biomedical applications of CDs-containing composites. While CDs so far have been widely used as biosensors and bioimaging agents, in the present study for the first time, we evaluate the osteoconductivity of CDs in poly (ε-caprolactone) (PCL)/polyvinyl alcohol (PVA) [PCL/PVA] nanofibrous scaffolds. Moreover, further studies were performed to evaluate egg shell-derived calcium phosphate (TCP3) and its cellular responses, biocompatibility and in vitro osteogenesis. Scaffolds were fabricated by simultaneous electrospinning of PCL with three different types of calcium phosphate, PVA and CDs. Fabricated scaffolds were characterized by Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), contact angle measurement and degradation assessment. SEM, the methyl thiazolyl tetrazolium (MTT) assay, and alkaline phosphatase (ALP) activity test were performed to evaluate cell morphology, proliferation and osteogenic differentiation, respectively. The results demonstrated that while the addition of just 1 wt% CDs and TCP3 individually into PCL/PVA nanocomposite enhanced ALP activity and cell proliferation rate (p < 0.05), the synergetic effect of CDs/TCP3 led to highest osteogenic differentiation and proliferation rate compared to other scaffolds (p < 0.05). Hence, CDs and PCL/PVA-TCP3 could serve as a potential candidate for bone tissue regeneration. © 2019 Elsevier B.V.
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