The Effect of 2% Alcohol Green Tea Extract on Healing Process of Open Wound in Male Mice



Yaghmayei P¹ ; Moshrefjavadi F² ; Nilforooshzade MA³ ; Mardani H⁴ ; Kadanejadian P² Show Affiliations
Source: Journal of Isfahan Medical School Published:2009

Abstract

Background: This research has been done with awareness of both positives effect of green tea, which is approved by researchers and the importance of treatment of opened skin wound. Methods: 32 male mice were divided into 4 different groups. First group was control and the others were experimental groups. Different dose of alcohol (50, 150 and 300 µl) were injected in experimental groups. After anaesthetizing the mice, skin wound is created on the back of the mice by a 6 mm punch. While the mice in observer group were treated by physiology serum, around the wound of the back of other mice was injected alcohol of green tea. The recovery progress of the wound (wound size and healing time) was measured in the 1st, 3rd, 7th and 15th days of study. Fibroblasts, macrophages, neutrophils and endothelial cells were evaluated in wound specimen in 3rd, 7th and 15th days of the study. Findings: There were no significant differences among these groups on 4th day of study. The degree of recovery on 7th day for observer group was (19.2 ± 10.2) for 2nd groups were (38.8 ± 17.9), for 3rd groups were (48.3 ± 17.7), for 4th were (60 ± 17.8) (P < 0.04). The degree of wound healing recovery at 15th day of the study was significantly different, in different groups: 25± 8.2 (group 1), 47.8 ± 17.5, (group 2), 66.5± 18.1 (group 3) and 76.5± 18.4 (group 4), (P < 0.01). The degrees of fibroblasts in different groups were as the following: group 1 (observer) (106 ± 13.8, 185.8 ± 12.1, 90 ± 10.5), group 2 (84 ± 19.7, 158.1 ± 14.8, 103.3 ± 12.9), group 3 (96.8 ± 22.9, 169.2 ± 16.5, 109.8 ± 10.8), and group 4 (99.8 ± 20.8, 173. 8 ± 12, 125 ± 11.9). Conclusion: Alcohol of green tea extracts speed up the wound healing process. We recommend more researches in the field of content enrichment, roots of administration and the effect of them. Examine the water and alcohol of green tea extract on different animals (chronic wounds and burnt) and finally on sick persons. © 2009, Isfahan University of Medical Sciences(IUMS). All rights reserved.