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Antimicrobial Activity and Wound Healing Effect of a Novel Natural Ointment: An in Vitro and in Vivo Study Publisher Pubmed



Khodabakhshi D1 ; Vaseghi G2 ; Mirzaee A3 ; Eskandarinia A1 ; Kharazi AZ1, 4
Authors
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Authors Affiliations
  1. 1. Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Hezarjreeb St., Isfahan, 8174673461, Iran
  2. 2. Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Hezarjreeb St., Isfahan, 8174673461, Iran
  3. 3. Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Hezarjreeb St., Isfahan, 8174673461, Iran
  4. 4. Applied Physiology Research Center, Isfahan University of Medical Sciences, Hezarjreeb St., Isfahan, 8174673461, Iran

Source: Journal of Wound Care Published:2023


Abstract

Infection and pathological disorders, such as cellular disorders, ischaemia, neuropathy and angiogenesis, are considered the most critical factors which cause a delay in the wound healing process in patients with diabetes. This study aimed to investigate the effect of an ointment based on ostrich oil containing honey, beeswax, and ethanolic extracts of Nigella sativa, propolis and Cassia angustifolia on the wound healing process of diabetic rats. Gas chromatography/mass spectrometry analysis showed caffeic acid and pinostrobin chalcone molecules present in propolis, giving antibacterial and antifungal properties to the compound. The antibacterial assessment showed the ointment had remarkable antibacterial activity against Staphylococcus aureus (8.6±0.28mm), Escherichia coli (9.4±0.31mm), Acinetobacter baumannii (7.2±0.23mm) and Pseudomonas aeruginosa (13.9±0.42mm). In vivo results showed the ointment significantly accelerated wound healing and increased collagen deposition compared with the control (p<0.05). Histopathology evaluation also showed hair follicles, sebaceous glands and vessels in the group that used the ointment. These results proved successful and diabetic wound healing was rapid. Therefore, it could be concluded that the fabricated ointment could be a suitable candidate for wound healing. Declaration of interest: This study was performed with the support of the Isfahan University of Medical Sciences. The authors have no conflicts of interest to declare. © 2023 MA Healthcare Ltd. All rights reserved.
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