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Fabrication and Morphological Characterization of Poly (3Hydroxy Butyrate)/Nano Hydroxyapetite Nanocomposite Scaffold for Bone Tissue Engineering Publisher



Radmehr M1 ; Sadeghi M1 ; Karbasi S2 ; Khorasani SN1 ; Saadat A3 ; Behnamghader A3
Authors
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Authors Affiliations
  1. 1. Department of Chemical Engineering, Isfahan University of Technology, Isfahan 8415683111, Iran
  2. 2. Department of Medical Physics and Biomedical Engineering, Isfahan University of Medical Sciences, Isfahan, University Street, Iran
  3. 3. Department of Biomedical Engineering, Islamic Azad University Science and Research Branch, Tehran, Iran

Source: IFMBE Proceedings Published:2010


Abstract

This study is focused on synthesis of nano-particle hydroxyapetite (nHA) via sol-gel method and fabrication of nanocomposite scaffold of Poly (3-hydroxybutyrate)/ nHA via solvent-casting and particulate leaching technique. The scaffold samples were prepared with 4 %wt of PHB/chloroform solution with the HA: PHB weight ratio of 5:95 and 10:90. To preventing of nHA agglomeration, ultrasonic device has been used. Preparation of HA nano particles was confirmed by X-Ray Diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis. Size of nHA crystals has been in nano range and affirmed by Scherrer equation from XRD results of nano-particles and nanocomposite scaffold. FTIR analysis of pure PHB scaffolds and its composite showed that the peak of carbonyl group is sharper than it for PHB/nHA nanocomposite. In addition, intensity of peaks belongs to PO for nHA, decrease extremely. These peaks shift to lower wave number, too. It seems some hydrogen bonding interaction between carbonyl group of PHB and hydroxyl group and PO of nHA. It is indicating the good interaction between nHA and PHB in the interface of nanocomposite for controlling size and morphology of nano particles. The porosity of the samples was measured by diffusion method. Scanning electron microscopy (SEM) was used for morphology study of prepared scaffolds and the size of nano-particles in polymer matrix. SEM results showed the porosity size in the range of 200-250μm. The interconnectivity of scaffold has been showed by the existing cavities on the wall of porosity that have seen in SEM image of porosity from cross section and top surface of scaffolds. Distribution of nHA in scaffold were investigated by Energy dispersive X-ray (EDX) and the results showed uniform distribution for nHA in polymer matrix. The obtained results suggest these newly developed PHB/nHA composite scaffolds may serve as a three-dimensional substrate in bone tissue engineering. © 2010 International Federation for Medical and Biological Engineering.
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