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Morphology and Degradation of Poly (3-Hydroxybutyrate)/ Nano-Hydroxyapatite Scaffold Used in Tissue Engineering



Radmehr M1 ; Karbasi S2 ; Sadeghi M1 ; Khorasani SN1 ; Saadat A3
Authors

Source: Journal of Isfahan Medical School Published:2012

Abstract

Background: Nanocomposites of biodegradable polymers and bioactive ceramics have high biocompatible and mechanical properties and are thus of high importance in bone tissue engineering. Among these nanocomposites, poly (3-hydroxybutyrate)/nano-hydroxyapatite (PHB/nHA) has favorable porosity. Methods: Nanocomposite scaffolds of PHB/nHA were prepared via solvent-casting and particulate leaching technique. NHA constituted 0-10% of weight of polymers. The porosity of the samples was measured by diffusion method. Scanning electron microscopy (SEM) was used to evaluate the morphology of prepared scaffolds and size of nano-particles in the polymer matrix. Distribution of nHA in scaffold was investigated by energy dispersive X-ray (EDX). Degradation of scaffolds was studied by SEM, Fourier transform infrared (FTIR) spectroscopy, and weighting samples before and after degradation in phosphatebuffered saline (PBS) solution. Findings: Porosity percentage was not decreased by increasing nHA content. Size of HA particles on wall of scaffold porosity was in nano-scale and distribution of nano particles in polymer matrix was uniform. Type of PHB/nHA scaffold degradation in PBS solution was bulk degradation. Conclusion: Prepared scaffolds had good interconnectivity. Size and percentage of porosity was acceptable for cell growth, attachment, and seepage in tissue engineering. Therefore, these new PHB/nHA nanocomposite scaffolds may serve as a 3D substrate in tissue engineering. © 2012, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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