Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran

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Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran Publisher

Shokoohi G^{1, 2} ; Badali H^{3, 4} ; Ahmadi B⁵ ; Satoh K⁶ ; Nouripoursisakht S⁷ ; Nikaeen M⁸ ; Gramishoar M⁹ ; Jalalizand N¹⁰ ; Kianipour S¹¹ ; Mirhendi H¹¹ ; Makimura K¹²

Show Affiliations

1. Department of Parasitology and Mycology, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
2. Zoonosis Research Center, Jahrom University of Medical Sciences, Jahrom, Iran
3. Invasive Fungi Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4. Department of Medical Mycology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5. Department of Medical Laboratory Sciences, Faculty of Paramedical, Bushehr University of Medical Sciences, Bushehr, Iran
6. General Medical Education and Research Center, Teikyo University, Tokyo, Japan
7. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
8. Department of Environmental Health Engineering, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran
9. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
10. National Institute of Health Research, Isfahan Health Research Station, Tehran University of Medical Sciences, Tehran, Iran
11. Department of Medical Parasitology and Mycology, School of Medicine and Reference Mycology Laboratory, Isfahan University of Medical Sciences, Isfahan, Iran
12. Medical Mycology, Graduate School of Medicine, Teikyo University, Tokyo, Japan

Source: Current Medical Mycology Published:2021

Abstract

Background and Purpose: The frequency and genetic diversity of black fungi in environmental and clinical settings have not been fully studied in Iran. This study aimed to identify and evaluate intra- and inter-species DNA sequence variation and also understand the phylogenetic relationships of melanized fungi and relatives isolated from different geographical regions of Iran. Materials and Methods: In total, 111 clinical and environmental strains of dematiaceous fungi were isolated, and their internal transcribed spacer ribosomal DNA (rDNA) regions were sequenced and analyzed. Results: An inter-species nucleotide sequence diversity rate of 1 to 464 nucleotides was observed between the species. Intra-species differences were found in the strains of Alternaria alternata, Cladosporium cladosporioides, Alternaria tenuissima, Curvularia spicifera, Aureobasidium pullulans, Curvularia hawaiiensis, Neoscytalidium dimidiatum, Alternaria terricola, Alternaria chlamydospora, Didymella glomerata, and Drechslera dematioidea by 0–59, 0–22, 0–4, 0–4, 0–3, 0–2, 0–2, 0–2, 0–2, 0–1, and 0–1 nucleotide, respectively. Conclusion: The internal transcribed spacer rDNA is useful for the discrimination of several taxa of dematiaceous fungi. However, a better understanding of the taxonomy of species of Alternaria requires a larger rDNA region or a library of other gene sequences. Copyright © 2021, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center.

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Style	Citing Format
MLA	Shokoohi G, et al.. "Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran." Current Medical Mycology, vol. 7, no. 3, 2021, pp. 1-8.
APA	Shokoohi G, Badali H, Ahmadi B, Satoh K, Nouripoursisakht S, Nikaeen M, Gramishoar M, Jalalizand N, Kianipour S, Mirhendi H, Makimura K (2021). Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran. Current Medical Mycology, 7(3), 1-8.
Chicago	Shokoohi G, Badali H, Ahmadi B, Satoh K, Nouripoursisakht S, Nikaeen M, Gramishoar M, et al.. "Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran." Current Medical Mycology 7, no. 3 (2021): 1-8.
Harvard	Shokoohi G et al. (2021) 'Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran', Current Medical Mycology, 7(3), pp. 1-8.
Vancouver	Shokoohi G, Badali H, Ahmadi B, Satoh K, Nouripoursisakht S, Nikaeen M, et al.. Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran. Current Medical Mycology. 2021;7(3):1-8.
BibTex	@article{ author = {Shokoohi G and Badali H and Ahmadi B and Satoh K and Nouripoursisakht S and Nikaeen M and Gramishoar M and Jalalizand N and Kianipour S and Mirhendi H and Makimura K}, title = {Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran}, journal = {Current Medical Mycology}, volume = {7}, number = {3}, pages = {1-8}, year = {2021} }
RIS	TY - JOUR AU - Shokoohi G AU - Badali H AU - Ahmadi B AU - Satoh K AU - Nouripoursisakht S AU - Nikaeen M AU - Gramishoar M AU - Jalalizand N AU - Kianipour S AU - Mirhendi H AU - Makimura K TI - Isolation and Molecular Characterization of Clinical and Environmental Dematiaceous Fungi and Relatives From Iran JO - Current Medical Mycology VL - 7 IS - 3 SP - 1 EP - 8 PY - 2021 ER -

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