Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of Th17 Immune Responses of Recombinant Dna Vaccine Encoding Gra14 and Rop13 Genes Against Toxoplasma Gondii in Balb/C Mice Publisher Pubmed



Fatollahzadeh M1 ; Eskandarian A1 ; Darani HY1 ; Pagheh AS2 ; Ahmadpour E3, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
  3. 3. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Infection, Genetics and Evolution Published:2021


Abstract

Toxoplasma gondii, a worldwide opportunistic parasite, causes serious diseases in both humans and fetuses with defective immune systems. The development of an effective vaccine is urgently required to prevent and control the spread of toxoplasmosis, caused by the apicomplexan parasite Toxoplasma gondii which is one of the most damaging zoonotic diseases of global importance. Plasmid DNA vaccination is a promising procedure for vaccine development and following the previous studies, pcROP13 + pcGRA14 cocktail DNA vaccine was evaluated for Th17 immune responses. Four groups of BALB/c mice were immunized intramuscularly three times at 2-week intervals. Subsequently, the production of anti- T. gondii antibodies and serum levels of cytokines IL-17, and IL-22 were evaluated against the RH strain of T. gondii. In addition, both the reactive oxygen species (ROS) and parasite load were assessed using ELISA and Q-PCR, respectively. The results of this study showed that high levels of IgG were found in mice immunized with cocktail DNA vaccine (p < 0.05). The cytokines level of Th17, IL-17, and IL-22, increased remarkably in the immunized mice (p < 0.05). Also, significant induction (p < 0.05) was observed in ROS. In addition, immunization with pcROP13 + GRA14 resulted in a considerable decrease in parasite load compared to the control groups (p < 0.05). Based on the results, the pcROP13 + GRA14 cocktail DNA vaccine induced Th17 related cytokines and decreased the parasite load in spleen and brain tissues. Hence, pcGRA14 + pcROP13 cocktails are suitable candidates for DNA-based vaccines and due to the development of protective immune responses against T. gondii infection, future studies may yield promising results using these antigens in vaccine design. © 2021
Related Docs
View other Related Docs
1. A Systematic Review of Toxoplasma Gondii Antigens to Find the Best Vaccine Candidates for Immunization, Microbial Pathogenesis (2019)
2. Immunological Evaluation of a Novel Multi-Antigenic Dna Vaccine Encoding Sag1, Sag3, Mic4, Gra5, Gra7, Ama1and Bag1 Against Toxoplasma Gondii in Balb/C Mice, Experimental Parasitology (2023)
3. A Systematic Review on the Role of Gra Proteins of Toxoplasma Gondii in Host Immunization, Journal of Microbiological Methods (2019)
4. Toxoplasmosis and Symptoms Severity in Patients With Covid-19 in Referral Centers in Northern Iran, Journal of Parasitic Diseases (2023)
5. Human Anti-Toxoplasma Antibodies Attach Strongly to Breast Cancer Cells, International Journal of Cancer Management (2023)
6. Detection of Toxoplasma Parasitemia by Pcr: Does It Correlate With Igg and Igm Antibody Titers?, Iranian Journal of Immunology (2006)
7. Cloning, Expression and Characterization of Toxoplasma Gondii P35 Protein in E. Coli, Yakhteh (2006)
8. Detection of Toxoplasma Gondi in Acute and Chronic Phases of Infection in Immunocompromised Patients and Pregnant Women With Real-Time Pcr Assay Using Taqman Fluorescent Probe, Iranian Journal of Parasitology (2018)
Experts (# of related papers)
View all Related Experts
Seyed Hossein Hejazi (11)
Hossein Yousofi Darani (8)
Other Related Docs
9. The Seroprevalence of Anti-Toxoplasma Gondii Igg and Igm Antibodies in Serum Samples Referred to Educational Hospitals of Abadan, Journal of Isfahan Medical School (2022)
10. Effects of Toxoplasma Gondii and Toxocara Canis Antigens on Wehi-164 Fibrosarcoma Growth in a Mouse Model, Korean Journal of Parasitology (2009)
11. Rhomboid Antigens Are Promising Targets in the Vaccine Development Against Toxoplasma Gondii, EXCLI Journal (2019)
12. Low Titer of Antibody Against Toxoplasma Gondii May Be Related to Resistant to Cancer, Journal of Cancer Research and Therapeutics (2015)
13. High Occurrence of Toxoplasma Gondii Infection Among Blood Donors in Ardabil Province As Main Focus of Zoonotic Visceral Leishmaniosis, Northwestern Iran, Annals of Parasitology (2021)
14. The Gene Expression Level of Ifn-Γr1 and Ifn-Γr2 in a Murine Model Treated With Toxoplasma Gondii and Its Products, Gastroenterology and Hepatology from Bed to Bench (2016)
15. Comparison of the Re-529 Sequence and B1 Gene for Toxoplasma Gondii Detection in Blood Samples of the At-Risk Seropositive Cases Using Uracil Dna Glycosylase Supplemented Loop-Mediated Isothermal Amplification (Udg-Lamp) Assay, Microbial Pathogenesis (2020)
16. Signs of the Presence of Th17 Cells in Chronic Periodontal Disease, Journal of Periodontal Research (2012)
17. Anti-Toxoplasma Gondii Antibodies Attach to Mouse Cancer Cell Lines But Not Normal Mouse Lymphocytes, Biomedical Reports (2019)
18. Study of Serological Toxoplasmosis and Risk Factors Associated With Infection in Women Referred to Labs of Northern Tehran, Iran, Journal of Isfahan Medical School (2013)
19. Knocking Down the Expression of the Molecular Motors, Myosin A, C and F Genes in Toxoplasma Gondii to Decrease the Parasite Virulence, Experimental Parasitology (2023)
20. Prevalence of Toxoplasma Infection in Veterinary Laboratory Sciences Students Comparing to Ordinary People: A Case–Control Study, Journal of Parasitic Diseases (2016)
21. Prevalence of Toxoplasma Gondii and the Level of Il8 in Patients With Celiac Disease, Koomesh (2016)
22. Seroprevalence of Toxoplasma Infection in Individuals Occupationally Exposed to Livestock and Raw Meat: A Case–Control Study, Veterinary Medicine and Science (2023)
23. Serological Evaluation of Anti-Toxoplasma Gondii Antibodies in Patients With Acute Leukemia and Lymphoma Through Chemotherapy, Iranian Journal of Parasitology (2020)
24. Seroepidemiology of Toxoplasma Gondii Infection in Pregnant Women in West Iran: Determined by Elisa and Pcr Analysis, Journal of Parasitic Diseases (2017)
25. Parasites As Potential Targets for Cancer Immunotherapy, Journal of Cancer Research and Clinical Oncology (2023)
26. Insights Into the Biochemical Features and Immunogenic Epitopes of Common Bradyzoite Markers of the Ubiquitous Toxoplasma Gondii, Infection, Genetics and Evolution (2021)
27. Prevalence of Coccidians and Helminthes Ova in Soil Samples From Public Places in Isfahan City, Iran, 2016, Journal of Isfahan Medical School (2017)
28. In Vitro and in Vivo Anti-Toxoplasma Activity of Dracocephalum Kotschyi Essential Oil, Food Science and Nutrition (2021)
29. Utility of Blood As the Clinical Specimen for the Diagnosis of Ocular Toxoplasmosis Using Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification and Real-Time Polymerase Chain Reaction Assays Based on Rep-529 Sequence and B1 Gene, BMC Infectious Diseases (2022)
30. Sero-Molecular Prevalence of Toxoplasmosis in Hemodialysis and Peritoneal Dialysis Patients in Markazi Province, Iran, Tropical Medicine and Health (2025)
31. Knocking Down of the Dhfr-Ts Gene in Toxoplasma Gondii Using Sirna and Assessing the Subsequences on Toxoplasmosis in Mice, Acta Tropica (2020)
32. Toxocara Seroprevalence and Associated Risk Factors Among Ilam Children, West of Iran, Archives of Pediatric Infectious Diseases (2019)
33. The Prevalence Rate of Infection to Toxoplasma Gondii in Domestic and Industrial Breeding Poultry in Isfahan City, Iran, 2020, Journal of Isfahan Medical School (2021)
34. Evaluation of Antinuclear Antibodies in Pregnant Women With Abortion With Toxocariasis and Toxoplasmosis in Iran; [Iran’Da Toksokariyaz Ve Toksoplazmozlu Dusuk Yapan Gebelerde Antinukleer Antikorlarin Degerlendirilmesi], Turk Hijyen ve Deneysel Biyoloji Dergisi (2022)
35. Seroprevalence of Toxoplasmosis in Mentally Retarded Patients in Iranian Rehabilitation Centers, Journal of Parasitic Diseases (2015)
36. Seroprevalence of Toxoplasma Gondii and Toxocara Spp. Infections Among Pregnant Women With and Without Previous Abortions in the West of Iran, Journal of Obstetrics and Gynaecology Research (2020)
37. Exposure to Leishmania Major Modulates the Proportion of Cd4+ T Cells Without Affecting Cellular Immune Responses, Microbiology and Immunology (2007)
38. Comparison of Eight Cell-Free Media for Maintenance of Toxoplasma Gondii Tachyzoites, Iranian Journal of Parasitology (2016)
39. Seroprevalence of Toxoplasmosis in Kermanshah City, West of Iran, Annals of Parasitology (2021)
40. Prevalence of Toxoplasma Gondii in Hiv-Infected Individuals in Iran: A Systematic Review and Meta-Analysis, Current HIV Research (2023)
41. Frequency of Blood-Tissue Parasitic Infections in Patients With Multiple Sclerosis, As Compared to Their Family Members, International Journal of Preventive Medicine (2014)
42. Toxoplasma Gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look Through an in Silico Prism, BioMed Research International (2021)
43. A Simple and Innovative Method for Cell Membrane Passing the Small Interfering Rna (Sirna) in Eukaryotic Cells; Toxoplasma Gondii As an Experience, Journal of Isfahan Medical School (2017)
44. Toxoplasma Serology Status and Risk of Miscarriage, a Case-Control Study Among Women With a History of Spontaneous Abortion, International Journal of Fertility and Sterility (2019)
45. New Insight to Il-23/Il-17 Axis in Iranian Infected Adult Patients With Gastritis : Effects of Genes Polymorphisms on Expression of Cytokines, Acta Gastro-Enterologica Belgica (2015)
46. Parasites and Cancers: Parasite Antigens As Possible Targets for Cancer Immunotherapy, Future Oncology (2012)
47. Seroepidemiology of Toxoplasma Gondii Infection in Isfahan Province, Central Iran: A Population Based Study, Journal of Research in Medical Sciences (2011)
48. Evaluation of Toxoplasma Gondii Infection in Western Iran: Seroepidemiology and Risk Factors Analysis, Tropical Medicine and Health (2020)
49. Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (Cdpk7) Showed Potential Targets for Toxoplasma Gondii Vaccine, Journal of Parasitology Research (2021)
50. Parasites and Immunotherapy: With or Against?, Journal of Parasitic Diseases (2016)