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Anti-Toxoplasma Gondii Antibodies Attach to Mouse Cancer Cell Lines But Not Normal Mouse Lymphocytes Publisher



Mohamadi F1 ; Shakibapour M1 ; Sharafi SM2 ; Andalib AR3 ; Tolouei S1 ; Darani HY1, 4
Authors
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Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, 8179498861, Iran
  2. 2. Environment Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan, 8179498861, Iran
  3. 3. Department of Immunology, Isfahan University of Medical Sciences, Isfahan, 8179498861, Iran
  4. 4. Cancer Prevention Research Centre, Isfahan University of Medical Sciences, Isfahan, 8179498861, Iran

Source: Biomedical Reports Published:2019


Abstract

Toxoplasma gondii (T. gondii) is prevalent intracellular parasite and a cause of worldwide infection in the human population. An inhibitory effect of this parasite on cancer growth has been demonstrated in cell culture and animal models. To determine whether the anticancer activities of T. gondii are associated with host immune response, in the current study the reactivity of anti‑T. gondii antiserum with the surface of cancer cell lines was investigated. Anti‑T. gondii antibodies were raised in rabbit and the reaction of this antiserum in comparison with other anti‑parasite antisera (anti‑T. vaginalis, anti‑hydatid cyst fluid, anti‑protoscolices antigens) with mouse melanoma or breast cancer cells lines was investigated using flow cytometry. Anti‑T. gondii antiserum reacted markedly with the surface of mouse melanoma and breast cancer cells, and less so with the normal mouse spleen lymphocytes. Meanwhile, the other anti‑parasite antisera did not react strongly with the surface of cancer cells compared with normal mouse spleen lymphocytes. In summary, it has been demonstrated herein that anti‑T. gondii antiserum may selectively react with the surface of mouse cancer cells but not with normal mouse spleen lymphocytes. Therefore, further study on anti‑Toxoplasma antibodies may be useful for directing the application of selective drug delivery in cancer treatment. © 2019, Spandidos Publications. All rights reserved.
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