Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of the Effects of Photodynamic Therapy With Methylene Blue on Different Candida Species in Vitro Publisher



Khozeimeh F1 ; Tavangar A2 ; Abyaneh MR3 ; Amini M4 ; Ghayour M5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Oral and Maxillofacial Medicine, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Dental Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Mycology, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Oral Medicine Specialist, Dental Material Research Center, Department of Oral and Maxillofacial Medicine, Isfahan, Iran
  5. 5. Pediatric Dentist, Pediatric Department, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Lasers in Medical Sciences Published:2023


Abstract

Introduction: Oral candidiasis is the most prevalent opportunistic infection of the oral cavity. The most common cause of this infection is Candida albicans. Considering the side effects of conventional antifungal therapies, this study aimed to evaluate the efficacy of photodynamic therapy with new methylene blue (a photosensitizer) in inhibiting the growth of C. albicans, C. tropicalis, C. glabrata, and C. krusei in vitro. Methods: In this experimental study, 200 samples of standard suspension (0.5 McFarland) were prepared from C. albicans, C. tropicalis, C. glabrata, and C. krusei (50 samples from each species). The samples of each species were divided into five groups (n = 10), including photodynamic therapy with a photosensitizer, with or without laser irradiation, nystatin treatment, laser therapy, and control. Next, cultivation of samples was performed on Sabouraud dextrose agar, and the colony-forming units were determined after 24 hours of incubation at 37 °C. Data were analyzed in SPSS version 22 by means of the Kruskal-Wallis test (P < 0.05). Results: The most sensitive and resistant species to nystatin therapy were C. glabrata and C. krusei, respectively. On the other hand, C. krusei was the most sensitive species to photodynamic therapy, and C. glabrata was the most resistant type to this treatment. The highest therapeutic effectiveness was attributed to nystatin therapy, although photodynamic therapy was also effective. Laser therapy was recognized as the least effective method. Conclusion: Photodynamic therapy with new methylene blue, as a suitable adjunct therapy, can be effective in the management of candidiasis. It may also be a potential novel treatment for immunocompromised patients with oral candidiasis. © (2023), (Laser Application in Medical Sciences Research Center). All Rights Reserved.
Related Docs
View other Related Docs
1. Evaluation and Comparison of Antifungal Effect of Voriconazole With Nystatin on Candida Species Derived From Neoplastic Patients Undergoing Maxillofacial Radiotherapy, Dental Research Journal (2023)
2. Overview on the Infections Related to Rare Candida Species, Pathogens (2022)
3. Synergistic Activity Between Echinophora Platyloba Dc Ethanolic Extract and Azole Drugs Against Clinical Isolates of Candida Albicans From Women Suffering Chronic Recurrent Vaginitis, Journal de Mycologie Medicale (2014)
4. Species Distribution and Susceptibility Profiles of Oral Candidiasis in Hematological Malignancy and Solid Tumor Patients, Brazilian Journal of Microbiology (2023)
5. Antifungal Activity of Anethum Graveolens Extract and Atrovastatin Against Candida Species Compared to Fluconazole, Journal of Isfahan Medical School (2015)
6. Synthesis and Characterization of Antifungal Nanocomposite Agsio 2Polymethyl Methacrylate, European Journal of Dentistry (2022)
7. The Evaluation of the Overexpression of the Erg-11, Mdr-1, Cdr-1, and Cdr-2 Genes in Fluconazole-Resistant Candida Albicans Isolated From Ahvazian Cancer Patients With Oral Candidiasis, Journal of Clinical Laboratory Analysis (2022)
8. Comparison of Oral Candidiasis Characteristics in Head-And-Neck Cancer Patients Before and During Radiotherapy, Dental Research Journal (2023)
Experts (# of related papers)
View all Related Experts
Parvin Dehghan (12)
Rasoul Mohammadi (9)
Other Related Docs
9. The Frequency of Oral Candidiasis in Children and Adolescents With Leukemia in Isfahan Province, Iran, Journal of Isfahan Medical School (2017)
10. Antifungal Activity of Almond (Prunus Amygdalus) Hull Extracts Against Clinical Isolates of Candida Albicans, Current Medical Mycology (2024)
11. Evaluation of the Antifungal Effect of Fluconazole Separately and With Coriander Essential Oil on Candida Species Isolated From the Mouth of Hiv-Positive Individuals, Scientific Journal of Kurdistan University of Medical Sciences (2023)
12. Epidemiology and Antifungal Susceptibility of Candida Species Isolated From 10 Tertiary Care Hospitals in Iran, Microbiology Spectrum (2022)
13. The Matching Rate of the Frequency of the Isolated Candida Species Calculated by Morphological and Molecular Methods in Patients With Vaginitis, Journal of Isfahan Medical School (2017)
14. The Effects of Nutraceuticals and Herbal Medicine on Candida Albicans in Oral Candidiasis: A Comprehensive Review, Advances in Experimental Medicine and Biology (2021)
15. Caspofungin-Non-Susceptible Candida Orthopsilosis Isolated From Onychomycosis in Iran, Iranian Journal of Public Health (2017)
16. Anti Candidal Activity of Echinophora Platyloba Against Candida Albicans and Comparison With Amphotricin, Journal of Medicinal Plants (2009)
17. Candida Africana Vulvovaginitis: Prevalence and Geographical Distribution, Journal de Mycologie Medicale (2020)
18. Antimicrobial Photodynamic Therapy With Dendrosomal Curcumin and Blue Laser Against Porphyromonas Gingivalis, Photodiagnosis and Photodynamic Therapy (2023)
19. Candida Palmioleophila Candidemia and Bacterial Co-Infection in a 3-Month-Old Infant With Biliary Atresia, Frontiers in Cellular and Infection Microbiology (2023)
20. Multicenter Identification and Antifungal Susceptibility Patterns of Candida Species Isolated From Clinical Samples, Jundishapur Journal of Microbiology (2017)
21. Evaluation of the Therapeutic Effect of 15 Ppm Silver Nanoparticle Spray Compared to Clotrimazole 1% on Candida Vaginitis: A Randomized Controlled Clinical Trial, Journal of Family and Reproductive Health (2023)
22. Identification of Candida Species and Antifungal Susceptibility in Cancer Patients With Oral Lesions in Ahvaz, Southern West of Iran, Advanced Biomedical Research (2020)
23. Study of Aqueous and Alcoholic Extract of the Melissa Officinalis Effect on Candida Albicans, Candida Glabrata and Candida Krusei, Journal of Military Medicine (2017)
24. Antifungal Activity of Zingiber Officinale Rosc. Essential Oil Against Fluconazole Resistant Vaginal Isolates of Candida Albicans, Journal of Medicinal Plants (2007)
25. Antifungal Susceptibility Testing of Candida Species Isolated From the Immunocompromised Patients Admitted to Ten University Hospitals in Iran: Comparison of Colonizing and Infecting Isolates, BMC Infectious Diseases (2017)
26. Species Distribution and Antifungal Susceptibility Profile of Candida Isolates From Blood and Other Normally Sterile Foci From Pediatric Icu Patients in Tehran, Iran, Medical Mycology (2020)
27. Role of Laser or Photodynamic Therapy in Treatment of Denture Stomatitis: A Systematic Review, Journal of Prosthetic Dentistry (2018)
28. Frequency of Candida Species Isolated From the Oral Cavity of Hiv-Infected Patients Referring to Behavioral Disease Counseling Center of Isfahan in 2017-2018, Scientific Journal of Kurdistan University of Medical Sciences (2019)
29. Identification of Candida Species in Oral Cavity of Smokers and Nonsmokers, Journal of Isfahan Medical School (2016)
30. Detection of Candida Albicans in Oral Squamous Cell Carcinoma by Fluorescence Staining Technique, Dental Research Journal (2015)
31. Antifungal Effect of Atorvastatin in Comparison With Fluconazole on Candida Species Isolated From Patients Undergoing Head‑And‑Neck Radiotherapy, Dental Research Journal (2024)
32. Antifungal Effect of Echinophora Platyloba Essence Against the Candida Species Isolated From Vulvovaginal Candidiasis, Compared With Fluconazole, Journal of Isfahan Medical School (2014)