Chitosan Microparticles Loaded With Exotoxin a Subunit Antigen for Intranasal Vaccination Against Pseudomonas Aeruginosa: An in Vitro Study Publisher



Taranejoo S¹ ; Janmaleki M¹ ; Rafienia M² ; Kamali M³ ; Mansouri M³
Source: Carbohydrate Polymers Published:2011

Abstract

Chitosan microparticles (CMs) were prepared with tripolyphosphate by spray-drying. Effects of polymer molecular weight, sonication power, cross-linking time and concentration of TPP on release profiles of catalytic or third domain pseudomonas exotoxin A (PEIII) and morphology of CMs were evaluated. The mean particle sizes of CMs were in the range from 1.09-1.46 μm and antigen loading efficiencies were more than 59%. As the molecular weight of chitosan increased, microparticles had a more spherical shape and a smooth surface. An increase in sonication power and decrease in cross-linking time resulted microparticles morphology changes. Approximately 60-80% of PEIII released from microparticles within the first few hours. The release of antigen is increased significantly by raising the sonication power more than 45 W. When the cross-linking time extended from 15 to 60 min, the release of PEIII significantly reduced. The release of PEIII from the microparticles increased when concentration of TPP was raised. © 2010 Elsevier Ltd. All rights reserved.