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The Relationship of Oocyte and Sperm Membrane Binding Protein With the Success of Intrauterine Insemination



Seifali R1 ; Aboutorabi R2 ; Shahverdi A3 ; Ebrahimi B3 ; Mazani F1 ; Esmaili V3
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Anatomical Sciences, School of Medicine and Infertility Laboratory, Shahid Beheshti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, The Academic Center for Education, Culture and Research (ACECR), Tehran, Iran

Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Fertilization in mammals is dependent on successful connection between the sperm and oocyte plasma membrane. Gametes binding largely been studied in mice and the crucial role of CD9 (Cluster of differentiation 9), a member of tetraspanin family, is shown in gametes binding in mice. CD9 found in the inner membrane of the acrosome; and only a small amount of CD9 is expressed on the plasma membrane of mid piece. Inner sperm membrane proteins play an important role in the fertilization process. The aim of this study was to obtain correlation between the percentages of CD9- positive sperm with outcome of intrauterine insemination (IUI). Methods: A total of 120 semen samples from infertile couples referred to Royan Institute (Isfahan, Iran) who were undergoing intrauterine insemination treatment were collected. Semen parameters such as motility, morphology, sperm concentration and viscosity were analyzed according to World Health Organization (WHO) 2010 guidelines. Sperm washed and stained via using anti-CD9 monoclonal antibody. The samples were analyzed via flow cytometry technique. Findings: CD9 was detected only in 19% of mature sperms. The result of the receiver operating characteristic (ROC) area under the curve was equal to 0.446; therefore, CD9 was not a good variable predictor for fertility status (P = 0.482). Conclusion: There was not a significant correlation between the CD9 expression and fertilization in patients underwent intrauterine insemination. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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