Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Spermatozoa Induce Maternal Mononuclear Cells for Production of Antibody With Cytotoxic Activity on Paternal Blood Mononuclear Cells Publisher



Sereshki N1, 2 ; Andalib A1 ; Toghyani M1 ; Motedayyen H3 ; Hesamian MS1 ; Rezaei A1 ; Wilkinson D4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Asadabad School of Medical Science, Asadabad, Iran
  3. 3. Autoimmune Diseases Research Centre, Kashan University of Medical Sciences, Kashan, Iran
  4. 4. University of Aberdeen, United Kingdom

Source: Cell Journal Published:2021


Abstract

Objective: The maternal immune response to paternal antigens is induced at insemination. We believe that pregnancy protective alloantibodies, such as anti-paternal cytotoxic antibody (APCA), may be produced against the paternal antigens in the context of stimulated immunity at insemination and that they increase during pregnancy. APCA is necessary for pregnancy. It is directed towards paternal human leucocyte antigens (HLAs) and has cytotoxic activity against paternal leucocytes. The present study aims to determine whether APCA is produced by the maternal peripheral blood mononuclear cells (PBMCs) in contact with the husband's spermatozoa and to evaluate the relation of APCA production with HLA class I and II expressions by spermatozoa in fertile couples. Materials and Methods: This cross-sectional study included 30 fertile couples with at least one child. The maternal PBMCs were co-cultured with the husband's spermatozoa and the supernatant was assessed for the presence of IgG by ELISA. Cytotoxic activity of the supernatant on the husband's PBMCs was assessed by the complement-dependent cytotoxicity (CDC) assay. Results: IgG was produced in all co-cultures, and the mean level of supernatant IgG was 669 ng/ml. The cytotoxic activity of the supernatant was observed in all the supernatant obtained from the co-cultures. The mean percentage of APCA in supernatant was 73.93%. Conclusion: Based on the results of this study it can be concluded that APCA may be a natural anti-sperm antibody (ASA), which can be produced during exposure to spermatozoa and may have some influence before pregnancy. Further research is required to determine the role of APCA before pregnancy. © 2021 Royan Institute (ACECR). All rights reserved.
Related Docs
View other Related Docs
1. The Expression of Human Leukocyte Antigen by Human Ejaculated Spermatozoa, Molecular Genetics and Genomic Medicine (2019)
2. Lack of Anti-Paternal Cytotoxic Antibody Production in the Presence of Spermatozoa in Recurrent Spontaneous Abortion Couples in Contrast to Fertile Couples: A Pilot Study, Clinical and Experimental Obstetrics and Gynecology (2021)
3. Can the Decreased Expression of Human Leukocyte Antigen Class I and Ii by Spermatozoa Lead to Recurrent Spontaneous Abortion?, Iranian Journal of Pathology (2020)
4. The Effect of Probiotics on Immunogenicity Of Spermatozoa In Couples Suffering From Recurrent Spontaneous Abortion, BMC Immunology (2022)
5. Cd5 Expression by Human Ejaculated Spermatozoa, Middle East Fertility Society Journal (2022)
6. Decreased Toll-Like Receptor (Tlr) 2 and 4 Expression in Spermatozoa in Couples With Unexplained Recurrent Spontaneous Abortion (Ursa), Iranian Journal of Allergy, Asthma and Immunology (2019)
7. Effects of Failed Oocyte Activation and Sperm Protamine Deficiency on Fertilization Post-Icsi, Reproductive BioMedicine Online (2007)
8. Effects of Temperature and Storage Time on the Motility, Viability, Dna Integrity and Apoptosis of Processed Human Spermatozoa, Andrologia (2020)
Experts (# of related papers)
View all Related Experts
Abbas Rezaei (6)
Shahnaz Razavi (5)
Other Related Docs
9. Variations in T-Helper 17 and Regulatory T Cells During the Menstrual Cycle in Peripheral Blood of Women With Recurrent Spontaneous Abortion, International Journal of Fertility and Sterility (2014)
10. Effect of Sperm Dna Damage and Sperm Protamine Deficiency on Fertilization and Embryo Development Post-Icsi, Reproductive BioMedicine Online (2005)
11. Comparing the Roles of Sperm Chromatin Integrity and Apoptosis in Intrauterine Insemination Outcomes of Couples With Mild Male and Female Factor Infertility, Journal of Reproduction and Infertility (2014)
12. Cd33 As a Leukocyte-Associated Marker Expressed on Human Spermatozoa, BMC Research Notes (2023)
13. Progesterone-Induced Blocking Factor (Pibf) Influences the Expression of Membrane Progesterone Receptors (Mprs) on Peripheral Cd4+ T Lymphocyte Cells in Normal Fertile Females, Hormones (2021)
14. The Relationship of Oocyte and Sperm Membrane Binding Protein With the Success of Intrauterine Insemination, Journal of Isfahan Medical School (2014)
15. Evaluation of the Leptin Receptor in Human Spermatozoa, Reproductive Biology and Endocrinology (2010)
16. Can Sperm Protamine Deficiency Induce Sperm Premature Chromosomal Condensation?, Andrologia (2006)
17. Effect of Human Sperm Mtt Viability Test on Outcome of Intracytoplasmic Sperm Injection, Yakhteh (2006)
18. Evaluation of Fas Positive Sperm and Complement Mediated Lysis in Subfertile Individuals, Journal of Assisted Reproduction and Genetics (2010)
19. Sperm Dna Damage and Its Relation With Leukocyte Dna Damage, Reproductive Toxicology (2010)
20. T-Helper (1) Cytokines Increase During Early Pregnancy in Women With a History of Recurrent Spontaneous Abortion, Medical Science Monitor (2002)