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Experimental and Theoretical Investigations of Dy(Iii) Complex With 2,2′-Bipyridine Ligand: Dna and Bsa Interactions and Antimicrobial Activity Study Publisher Pubmed



Arameshboroujeni Z1, 2 ; Jahani S3 ; Khorasanimotlagh M2 ; Kerman K4 ; Aramesh N5 ; Asadpour S6 ; Noroozifar M4
Authors
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Authors Affiliations
  1. 1. Department of Clinical Laboratory, AlZahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Chemistry, University of Sistan and Baluchestan, Zahedan, Iran
  3. 3. Nano Bioeletrochemistry Research Center, Bam University of Medical Sciences, Bam, Iran
  4. 4. Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto, ON, Canada
  5. 5. Department of Chemistry, Faculty of Sciences, Yasouj University, Yasouj, Iran
  6. 6. Department of Chemistry, Faculty of Sciences, Shahrekord University, Shahrekord, Iran

Source: Journal of Biomolecular Structure and Dynamics Published:2020


Abstract

In this study, the interactions of a novel metal complex [Dy(bpy)2Cl3.OH2] (bpy is 2,2'-bipyridine) with fish salmon DNA (FS-DNA) and bovine serum albumin (BSA) were investigated by experimental and theoretical methods. All results suggested significant binding between the Dy(III) complex with FS-DNA and BSA. The binding constants (Kb), Stern-Volmer quenching constants (KSV) of Dy(III)-complex with FS-DNA and BSA at various temperatures as well as thermodynamic parameters using Van’t Hoff equation were obtained. The experimental results from absorption, ionic strength, iodide ion quenching, ethidium bromide (EtBr) quenching studies and positive ΔH˚ and ΔS˚ suggested that hydrophobic groove-binding mode played a predominant role in the binding of Dy(III)-complex with FS-DNA. Indeed, the molecular docking results for DNA-binding were in agreement with experimental data. Besides, the results found from experimental and molecular modeling indicated that the Dy(III)-complex bound to BSA via Van der Waals interactions. Moreover, the results of competitive tests by phenylbutazone, ibuprofen, and hemin (as a site-I, site-II and site-III markers, respectively) considered that the site-III of BSA is the most possible binding site for Dy(III)-complex. In addition, Dy(III) complex was concurrently screened for its antimicrobial activities. The presented data provide a promising platform for the development of novel metal complexes that target nucleic acids and proteins with antimicrobial activity. Communicated by Ramaswamy H. Sarma. © 2019 Informa UK Limited, trading as Taylor & Francis Group.
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