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Cycloartane Triterpenoids From Euphorbia Macrostegia With Their Cytotoxicity Against Mda-Mb48 and Mcf-7 Cancer Cell Lines



Baniadam S1 ; Rahiminejad MR2 ; Ghannadian M3 ; Saeidi H2 ; Ayatollahi AM4 ; Aghaei M5
Authors
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Authors Affiliations
  1. 1. Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Biology, University of Isfahan, Isfahan, Iran
  3. 3. Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Phytochemistry Research Center, Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Clinical Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Iranian Journal of Pharmaceutical Research Published:2014

Abstract

The dried plant was extracted with dichloromethane and after defatting with hexane, transferred repeatedly on silica columns using dichloromethane-hexane and ethyl acetate-hexane as mobile phases. Finally the fractions were purified by high performance liquid chromatography using a Pack-Sil column and hexane: Ethyl acetate as mobile phase. The structures of the isolated compounds included: cycloart-25-ene-3β, 24-diol (1), cycloart-23(Z)- ene-3β, 25-diol (2), cycloart-23(E)-ene-3β, 25-diol (3), and 24-methylene-cycloart-3β-ol (4) were elucidated by 13C- and 1H-NMR as well as IR and by the aid of mass fragmentation pattern and comparing with the literature. The biological effects of the compounds were done by the MTT assay on two different cancer cell lines including MDA-MB48 and MCF-7. Among these compounds, cycloart-23(E)-ene-3β,25-diol (3) was the most active compound on MDA-MB468 cell line (LD50 = 2.05 μgmL-1) and cycloart-23(Z)-ene-3β, 25-diol (2) was the most active compound on MCF-7 cell line (LD50 = 5.4 μgmL-1). © 2014 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services.
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