Inhibitors of Α-Synuclein Fibrillation and Oligomer Toxicity in Rosa Damascena: The All-Pervading Powers of Flavonoids and Phenolic Glycosides Publisher Pubmed



Otzen DE¹ ; Mohammadbeigi H¹ ; Ward JL² ; Eskandari H¹ ; Ghanadian M³ ; Noletodias C¹ ; Lomax C¹ ; Tawfike A¹ ; Christiansen G⁴ ; Sutherland DS¹ Show Affiliations
Source: ACS Chemical Neuroscience Published:2020

Abstract

There is an intense search for natural compounds that can inhibit the oligomerization and fibrillation of α-synuclein (α- Syn), whose aggregation is key to the development of Parkinson's disease (PD). Rosa damascena is a medicinal herb widely used in Middle Eastern food, ceremonies, and perfumes. The herb is known to contain many different polyphenols. Here we investigated the existence of α-Syn fibrillation inhibitors in R. damascena extract. Different HPLC fractions of the extract were assessed in α-Syn fibrillation and toxicity assays. The most active fractions led to the formation of more α-Syn oligomers but with less toxicity to SH-SY5Y cells, according to MTT and LDH assays. LC-MS analysis identified gallic acid, kaempferol 3-glucoside, kaempferol-3-O-β-rutinoside, and quercetin which were subsequently shown to be strong α-Syn fibrillation inhibitors. Our results highlight the benefits of R. damascena extract to combat PD at the population level. © 2020 American Chemical Society.