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Evaluation of Various Water Resources in Isfahan, Iran, for the Presence of Helicobacter Pylori Using Fluorescent Nested Polymerase Chain Reaction



Navabakbar FT1 ; Salehi R2
Authors
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Authors Affiliations
  1. 1. Department of Microbiology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: Helicobacter pylori infection is one of the most common infectious agents worldwide. However, origin and the mode of transmission of this bacterium have not been clearly explained. One of the most probable routs of Helicobacter pylori transmission is through water resources. The aim of this study was to evaluate the presence of Helicobacter pylori in tap waters, well waters and Zayandeh Rood River collected from various locations in Isfahan, Iran. Methods: Totally, 100 liters of water were collected from Zayandeh Rood River, wells and drinking water pipeline network in the north, west, south and west locations of Isfahan city. Collected waters first subjected to filteration through 0.25 μm, then, filters were washed with phosphate buffered saline (PBS) and the washed out PBS used for DNA extraction. For DNA extraction, QiAamp DNA Mini Kit was used, fluorescent nested polymerase chain reaction was used for detection of Helicobacter pylori genome using UreC, 16srRNA and adhesion gene specific primers. Findings: Our results showed Helicobacter pylori infection in Zayandeh Rood river, well waters and drinking water from eastern region of Isfahan. Conclusion: Using primers srRNA16, UreC, fluorescent nested polymerase chain reaction method and fluorochromes 5CY, very little number of bacteria were identified. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.
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