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Evaluating Helicobacter Pylori Flaa in Clinical Isolates From Alzahra Hospital, Isfahan, Iran



Madhi M1 ; Moghim S2 ; Pursina F2 ; Adibi P3 ; Khademi F1 ; Farajzadegan Z4 ; Ghasemian Safaei H2
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Gastroenterology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Community Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2013

Abstract

Background: This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori. Methods: One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain reaction (PCR) was performed using the designed primer. Findings: PCR products showed that all extracted DNA were positive for flaA gene. Conclusion: We found flaA gene to be present in all clinical isolates. As it is highly similar to standard Helicobacter pylori strains, it can be used to detect the mentioned bacteria.
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