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Evaluating Helicobacter Pylori Flaa in Clinical Isolates From Alzahra Hospital, Isfahan, Iran



Madhi M1 ; Moghim S2 ; Pursina F2 ; Adibi P3 ; Khademi F1 ; Farajzadegan Z4 ; Ghasemian Safaei H2
Authors

Source: Journal of Isfahan Medical School Published:2013

Abstract

Background: This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori. Methods: One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain reaction (PCR) was performed using the designed primer. Findings: PCR products showed that all extracted DNA were positive for flaA gene. Conclusion: We found flaA gene to be present in all clinical isolates. As it is highly similar to standard Helicobacter pylori strains, it can be used to detect the mentioned bacteria.
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