Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Fabrication and Optimization of Electrospun Polymeric Nanofibers Loaded With 5-Fluorouracil and Rosemary Extract Publisher Pubmed



Jamali R1 ; Ghassami E2 ; Dinani MS3 ; Ghasemi R4 ; Mirian M5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmaceutics, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmaceutics, Faculty of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Nano Biotechnology, Jabir Ibn Hayyan Institute, Technical and Vocational Training Organization, Isfahan, Iran
  5. 5. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Pharmaceutical Nanotechnology Published:2022


Abstract

Background: Topical 5-fluorouracil (5FU) is one of the most prescribed medications for different types of skin cancer; however, it is associated with drug resistance and adverse ef-fects. Rosemary extract has promising dose-dependent antitumor effects, as well as a synergistic effect in combination with 5-fluorouracil, besides sensitization of the 5-FU-resistant cells. Objective: Polymeric nanofibers loaded with 5FU and rosemary extract were optimized to com-bine both ingredients in one controlled release drug delivery system, aiming to enhance the efficacy while retaining the adverse effects. Methods: Polymeric nanofibers loaded with 5-FU and rosemary were fabricated via electrospinning technique. Design expert software was utilized to study the effect of independent variables, including polymer concentration, voltage, and feeding rate on the characteristics of the resulting nanofibers. Afterwards, the FTIR spectrum and release kinetic of the drug and extract from the optimized nanofibers and their cytotoxic effect against A375 cell line were investigated. Results: The formulation composed of 6.65% PVA electrospun at 1 mL.h-1 and 17.5kV was chosen as the optimum fabrication condition. The mean diameter of the optimized nanofibers was 755 nm. The drug and rosemary extract contents were 75.38 and 93.42%, respectively. The fabrication yield was 100%, bioadhesion force was 1.28 N, and bead abundance was 10 per field. The cytotoxicity of the optimized formulation was significantly higher than the control groups. Conclusion: According to the appropriate loading percentage, release efficiency and release kinetics, bioadhesion force, and cytotoxicity, these nanofibers could be further investigated as a topical treatment option to increase the efficacy of 5-FU. © 2022 Bentham Science Publishers.
Related Docs
View other Related Docs
1. Poly (Butylene Adipate-Co-Terephthalate) Electrospun Nanofibers Loaded With 5-Fluorouracil and Curcumin in Treatment of Colorectal Cancer Cells, Polymer Testing (2018)
2. Polycaprolactone–Gelatin Membranes in Controlled Drug Delivery of 5-Fluorouracil, Polymer Science - Series A (2020)
3. 5Fu-Loaded Pcl/Chitosan/Fe3o4 Core-Shell Nanofibers Structure: An Approach to Multi-Mode Anticancer System, Advanced Pharmaceutical Bulletin (2022)
4. Preparation, Optimization, and in Vitro–In Vivo Evaluation of Sorafenib-Loaded Polycaprolactone and Cellulose Acetate Nanofibers for the Treatment of Cutaneous Leishmaniasis, Drug Delivery and Translational Research (2023)
5. Polycaprolactone/Chitosan Blend Nanofibers Loaded by 5-Fluorouracil: An Approach to Anticancer Drug Delivery System, Polymers for Advanced Technologies (2018)
6. Doxorubicin Hydrochloride - Loaded Electrospun Chitosan/Cobalt Ferrite/Titanium Oxide Nanofibers for Hyperthermic Tumor Cell Treatment and Controlled Drug Release, International Journal of Biological Macromolecules (2018)
7. Controlled Release of Metronidazole Benzoate From Poly Ε-Caprolactone Electrospun Nanofibers for Periodontal Diseases, European Journal of Pharmaceutics and Biopharmaceutics (2010)
8. Incorporation of Magnetic Nax Zeolite/Dox Into the Pla/Chitosan Nanofibers for Sustained Release of Doxorubicin Against Carcinoma Cells Death in Vitro, International Journal of Biological Macromolecules (2019)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (20)
Ali Jahanian Najafabadi (5)
Other Related Docs
9. Pmma Nanofibers Containing Keplerate-Type Polyoxometalate and Metronidazole: Preparation and Wound-Healing Effect in a Rat Model, Journal of Drug Delivery Science and Technology (2022)
10. Transferrin-Targeted Poly(Butylene Adipate)/Terephthalate Nanoparticles for Targeted Delivery of 5-Fluorouracil in Ht29 Colorectal Cancer Cell Line, Journal of Bioactive and Compatible Polymers (2017)
11. Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs, Polymers (2022)
12. Novel Electrospun Polyvinyl Alcohol/Chitosan/Polycaprolactone-Diltiazem Hydrochloride Nanocomposite Membranes for Wound Dressing Applications, Emergent Materials (2024)
13. Implementing Taguchi Method to Analyze Electrospinning Parameters Influence on Mg-Doped Fluorapatite Nanoparticles-Poly (Ε-Caprolactone) Nanocomposite Scaffold (Mg-Fa Nps/Pcl) Properties, Polymers for Advanced Technologies (2020)
14. Antioxidant, Antimicrobial and Antiviral Properties of Herbal Materials, Antioxidants (2020)
15. Physical and Biological Properties of Blend-Electrospun Polycaprolactone/Chitosan-Based Wound Dressings Loaded With N-Decyl-N, N-Dimethyl-1-Decanaminium Chloride: An in Vitro and in Vivo Study, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2020)
16. Platelet Rich Fibrin Containing Nanofibrous Dressing for Wound Healing Application: Fabrication, Characterization and Biological Evaluations, Materials Science and Engineering C (2021)
17. Poly (Ε-Caprolactone) Incorporated Bioactive Glass Nanoparticles and Simvastatin Nanocomposite Nanofibers: Preparation, Characterization and in Vitro Drug Release for Bone Regeneration Applications, Chemical Engineering Journal (2013)
18. Investigating the Performance of Drug Delivery System of Fluconazole Made of Nano–Micro Fibers Coated on Cotton/Polyester Fabric, Journal of Materials Science: Materials in Medicine (2017)
19. Clinical Assessment of Novel Nanofibrous Dressings for Cutaneous Leishmaniasis: A Trial-Based Study, Polymers for Advanced Technologies (2024)
20. Recent Advances in Electrospun Protein Fibers/Nanofibers for the Food and Biomedical Applications, Advances in Colloid and Interface Science (2023)
21. The Thickness of Electrospun Poly (Ε-Caprolactone Nanofibrous Scaffolds Influences Cell Proliferation, International Journal of Artificial Organs (2009)
22. Mupirocin Loaded Core-Shell Pluronic-Pectin-Keratin Nanofibers Improve Human Keratinocytes Behavior, Angiogenic Activity and Wound Healing, International Journal of Biological Macromolecules (2023)
23. Rgd Peptide Grafted Polybutylene Adipate-Co-Terephthalate/Gelatin Electrospun Nanofibers Loaded With a Matrix Metalloproteinase Inhibitor Drug for Alleviating of Wounds: An In Vitro/In Vivo Study, Drug Development and Industrial Pharmacy (2020)
24. A Ternary Nanocomposite Fibrous Scaffold Composed of Poly(Ε-Caprolactone)/Gelatin/Gehlenite (Ca2al2sio7): Physical, Chemical, and Biological Properties in Vitro, Polymers for Advanced Technologies (2021)
25. Luteinizing Hormone-Releasing Hormone Targeted Poly(Methyl Vinyl Ether Maleic Acid) Nanoparticles for Doxorubicin Delivery to Mcf-7 Breast Cancer Cells, IET Nanobiotechnology (2016)
26. Fabrication and Evaluation of Hesperidin Loaded Polyacrylonitrile/Polyethylene Oxide Nanofibers for Wound Dressing Application, Journal of Biomaterials Science, Polymer Edition (2021)
27. Electrospun Core-Shell Polycaprolactone/ Chitosan Nanofibrous Composite With Enhanced Curcumin Loading Capacity for Wound Healing Applications, Materials Today Chemistry (2024)
28. Multifunctional Electrospun Polyvinyl Alcohol/Gellan Gum/Polycaprolactone Nanofibrous Membrane Containing Pentoxifylline to Accelerate Wound Healing, Polymer Bulletin (2023)
29. Comparison of the Antifungal Activity of Fluconazole- and Ketoconazole-Loaded Pcl/Pvp Nanofibrous Mat, Bulletin of Materials Science (2021)
30. Optimisation of Processing Variables Effective on Self-Assembly of Folate Targeted Synpronic-Based Micelles for Docetaxel Delivery in Melanoma Cells, IET Nanobiotechnology (2015)
31. Antimicrobial Synthetic and Natural Polymeric Nanofibers As Wound Dressing: A Review, Advanced Engineering Materials (2022)
32. Montelukast Incorporated Poly(Methyl Vinyl Ether-Co-Maleic Acid)/Poly(Lactic-Co-Glycolic Acid) Electrospun Nanofibers for Wound Dressing, Fibers and Polymers (2017)
33. Galactosylated Nanostructured Lipid Carriers for Delivery of 5-Fu to Hepatocellular Carcinoma, Journal of Liposome Research (2012)
34. Chemofunctionalization of Knitted Silk to Improve Interface Connection in a Nano/Micro Scaffold Based on Polycaprolactone-Chitosan-Multi-Walled Carbon Nanotube/Silk, International Journal of Biological Macromolecules (2024)
35. Core-Shell Nanofibers of Poly (Ε-Caprolactone) and Polyvinylpyrrolidone for Drug Delivery System, Materials Research Express (2019)
36. Production and Evaluation of Polyblend of Agar and Polyacrylonitrile Nanofibers for in Vitro Release of Methotrexate in Cancer Therapy, Fibers and Polymers (2015)
37. A Bifunctional Electrospun Nanocomposite Wound Dressing Containing Surfactin and Curcumin: In Vitro and in Vivo Studies, Materials Science and Engineering C (2021)
38. Plant Extracts: Antioxidant and Antimicrobial Properties and Their Effect of Nanoencapsulation, Nanotechnology in Herbal Medicine: Applications and Innovations (2023)
39. Platelet Rich Fibrin Containing Nanofibrous Dressing for Wound Healing Application: Fabrication, Characterization and Biological Evaluations, Biomaterials Advances (2022)
40. Fabrication and Characterization of a Novel Wound Dressing With Clindamycin Loaded Pva Nanoparticles for Acne Treatment, Fibers and Polymers (2022)
41. Preparation of an Injectable Doxorubicin Surface Modified Cellulose Nanofiber Gel and Evaluation of Its Anti-Tumor and Anti-Metastasis Activity in Melanoma, Biotechnology Progress (2018)
42. Hybrid Nano-Micro Scaffolds for Cartilage Tissue Engineering: Integrating Pcl-Dwjm-Mwcnts on Chemically Modified Silk Fibroin, Journal of Polymers and the Environment (2025)
43. Fabrication, Characterization, and Drug Release Study of Vitamin C–Loaded Alginate/Polyethylene Oxide Nanofibers for the Treatment of a Skin Disorder, Polymers for Advanced Technologies (2019)
44. Novel Multi-Layer Silica Aerogel/Pva Composite for Controlled Drug Delivery, Materials Research Express (2019)
45. Double-Walled Microspheres Loaded With Meglumine Antimoniate: Preparation, Characterization and in Vitro Release Study, Drug Development and Industrial Pharmacy (2014)
46. Anti-Leishmanial Effects of a Novel Biocompatible Non-Invasive Nanofibers Containing Royal Jelly and Propolis Against Iranian Strain of Leishmania Major (Mrho/Ir/75/Er): An In-Vitro Study, Journal of Arthropod-Borne Diseases (2023)