Alteration of Mir-21, Mir-433 and Mir-590 Tissue Expression Related to the Tgf-Β Signaling Pathway in Ulcerative Colitis Patients Publisher Pubmed



Naghdalipour M¹ ; Moradi N^{2, 3} ; Fadaei R⁴ ; Rezghi Barez S⁵ ; Sayyahfar S¹ ; Mokhtare M⁶ ; Fard TK⁷ ; Fallah S⁷ ; Esteghamati A¹
Source: Archives of Physiology and Biochemistry Published:2022

Abstract

Ulcerative colitis (UC) is an inflammatory disease, and studies have suggested a role for TGF-β signalling pathway in the pathogenesis of UC. In the present study, we evaluated expression of TGF-β signalling genes and their regulatory microRNAs in patients with UC and control subjects. The expression of TGF-β1, SMAD2, SMAD3, miR-21, miR-101, miR-433, and miR-590 were evaluated using real-time PCR in biopsy samples of the patients and controls. Results showed increased expression of TGF-β1 and SMAD3 in the patients compared to controls. In addition, miR-21 and miR-433 were found to be higher in the patients compared to controls; however, miR-590 was found to be lower. Moreover, miR-433 was demonstrated to have positive correlation with SMAD3 and TGF-β while miR-21 was positively correlated with TGF-β1. MiR-590 was negatively correlated with SMAD2 and SMAD3. Results of the present study suggested a role for TGF-β signalling pathway related microRNAs in pathogenesis of UC. © 2020 Informa UK Limited, trading as Taylor & Francis Group.