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Detection of Metallo-Beta Lactamases Among Carbapenem-Resistant Pseudomonas Aeruginosa Publisher



Sheikh AF1, 2 ; Rostami S1, 2 ; Jolodar A3 ; Tabatabaiefar MA4 ; Khorvash F5 ; Saki A6 ; Shoja S1 ; Sheikhi R1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  2. 2. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  3. 3. Department of Biomolecular and Biochemistry, School of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
  4. 4. Department of Genetic, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  5. 5. Nosocomial Infections Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Biostatistics and Epidemiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Source: Jundishapur Journal of Microbiology Published:2014


Abstract

Background: Carbapenems are important drugs used for the treatment of Pseudomonas aeruginosa infections, however metallo-β-lactamases (MBL) are able to efficiently hydrolyze these classes of drugs. Immediate detection of the MBL-producing P. aeruginosa is necessary in order to accurately treat infections caused by this organism.; Objectives: To determine the prevalence of MBL producing P. aeruginosa in burn and non-burn patients by two phenotypic tests and polymerase chain reaction (PCR) and to compare phenotypic tests with PCR.; Materials and Methods: A total of 223 non-duplicate strains of P. aeruginosa were collected from three teaching hospitals of Ahvaz, Iran. Antimicrobial susceptibility and minimum inhibitory concentrations (MICs) of carbapenems (imipenem, meropenem, doripenem and ertapenem) were determined by the Kirby-Bauer and E-test methods. Combined disk (CD) test, MBL E-test and PCR were performed for carbapenem-resistant P. aeruginosa isolates.; Results: Amongst all the P. aeruginosa isolates, 58.7% were resistant to imipenem while 31.8%, 13.5% and 74.4% were resistant to meropenem, doripenem and ertapenem, respectively. Amongst all the P. aeruginosa isolates, 44.4% were multidrug resistant and 13.45% were resistant to all of the carbapenems. The CD test with doripenem disk/750 μg ethylene diamine tetra acetic acid (EDTA) had the highest efficiency compared to the other phenotypic tests. blaIMP and blaVIM genes were detected in 11.7% and 0.4% of isolates, respectively. blaSPM and blaNDM genes were not observed.; Conclusions: Epidemiological and regional evaluation of MBL-producing P. aeruginosa through simple and inexpensive methods should be considered for effective treatment of carbapenem-resistant P. aeruginosa infections. © 2014 Ahvaz Jundishapur University of Medical Sciences.
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