Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas Aeruginosa Isolated Form Cystic Fibrosis Patient



Fazeli H^{1, 4} ; Akbari R^{1, 4} ; Moghim S^{1, 4} ; Asadian A² ; Faghihinia J³ ; Saneeyan H³ ; Narimani T^{1, 4} Show Affiliations
Source: Journal of Isfahan Medical School Published:2012

Abstract

Background: Cystic fibrosis (CF) is an autosomal recessive disorder due to genetic defect resulting cilliary function impairment. So that these patients are inclined to recurrent infection with opportunistic microorganism such as Pseudomonas aeruginosa. This organism is most important pathogen in cystic fibrosis (CF) which intend to colonize and form biofilm to confront immunsystem and therapeutic dosage of antimicrobial drug. These bacteria can mutate to survive and adapted to new to new condition. Colony morphotyping and antimicrobial drug resistant detecting in Pseudomonas aeruginosa can help treat of CF patient and prevent patient form being resource of resistant strain bacteria. The aim of this assay is detection of colony morphotype, antimicrobial resistant in Pseudomonas aeruginosa isolated form CF and urinary tract infection (UTI) patient, hospital personnel and environment. Methods: It is a cross sectional study. Samples wet were taken with swab in 59 CF patients (between 82-87 years), 27 UTI patient, 113 sample from hospital environment, 123 sample from 91 personnel (throat, hand, lab uniform) in azahra hospital. Isolated Pseudomonas aeruginosa form these samples were identified bacteriologic standard method. The isolates were investigated for morphotyping and antimicrobial drug resistance. Findings: 21 cases form 59 patients (%35.5), 25 cases 27 UTI patient (%92.5) were infected with Pseudomonas aeruginosa. Pseudomonas aeruginosa were isolated from 11 of 113 sample taken from hospital environment and 4 of the (123 sample) were taken 91 hospital personnel. From 21 Pseudomonas aeruginosa isolated from CF patient (35 male, 24 female and the ages between 2 month to 24 years), 9 isolates had mucoid form (%42) and the rate antibiotic resistance to Gentamicin, Amikacin, ciprofloxacin, piperacillin and ceftazidime were 9.5%, 9.5%, 14.2%, 19% and 86% respectively. Conclusion: The mucoid form of Pseudomonas aeruginosa was seen most frequently in CF patients comparing with other samples. In all groups, antimicrobial resistance to Ceftazidime and antimicrobial sensitivity to Amikacin were most frequent.