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Development of an Injectable Shear-Thinning Nanocomposite Hydrogel for Cardiac Tissue Engineering Publisher



Soltani S1 ; Emadi R1 ; Javanmard SH2 ; Kharaziha M1 ; Rahmati A3 ; Thakur VK4, 5 ; Lotfian S6
Authors
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Authors Affiliations
  1. 1. Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  2. 2. Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81746-73461, Iran
  3. 3. Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran
  4. 4. Biorefining and Advanced Materials Research Center, Scotland’s Rural College (SRUC), Kings Buildings, Edinburgh, EH9 3JG, United Kingdom
  5. 5. School of Engineering, University of Petroleum & Energy Studies (UPES), Uttarakhand, Dehradun, 248007, India
  6. 6. Faculty of Engineering, University of Strathclyde, Glasgow, G40LZ, United Kingdom

Source: Gels Published:2022


Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) offer a promising therapeutic method for cardiac tissue regeneration. However, to monitor the fate of MSCs for tissue repair, a better stem cell delivery carrier is needed. Developing a unique injectable and shear-thinning dual cross-linked hybrid hydrogel for MSC delivery for cardiac tissue engineering is highly desirable. This hydrogel was synthesised using guest: host reaction based on alginate-cyclodextrin (Alg-CD) and adamantane-graphene oxide (Ad-GO). Here, the role of macromere concentration (10 and 12%) on the MSC function is discussed. Our hybrid hydrogels reveal a suitable oxygen pathway required for cell survival. However, this value is strongly dependent on the macromere concentrations, while the hydrogels with 12% macromere concentration (2DC12) significantly enhanced the oxygen per-meability value (1.16-fold). Moreover, after two weeks of culture, rat MSCs (rMSCs) encapsulated in Alg-GO hydrogels expressed troponin T (TNT) and GATA4 markers. Noticeably, the 2DC12 hy-drogels enhance rMSCs differentiation markers (1.30-times for TNT and 1.21-times for GATA4). Overall, our findings indicate that tuning the hydrogel compositions regulates the fate of encapsulated rMSCs within hydrogels. These outcomes may promote the advancement of new multifunc-tional platforms that consider the spatial and transient guidelines of undifferentiated cell destiny and capacity even after transplantation for heart tissue regeneration. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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