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An Innovative Approach to Fabricate a Thermosensitive Melatonin-Loaded Conductive Pluronic/Chitosan Hydrogel for Myocardial Tissue Engineering Publisher



Torabi H1 ; Mehdikhani M1 ; Varshosaz J2, 3 ; Shafiee F4
Authors
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Authors Affiliations
  1. 1. Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran
  2. 2. Novel Drug Delivery Systems Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Applied Polymer Science Published:2021


Abstract

This study aimed at designing and fabrication of a novel injectable and thermosensitive melatonin-loaded pluronic/chitosan hydrogel containing gold nanoparticles (GNPs) and poly glycerol sebacate (PGS) for myocardial tissue engineering. The PGS nanoparticles were used as the melatonin (drug model) carrier. The gelation time, syringeability, stability, and swelling of the hydrogel were scrutinized. Rheological properties, chemical composition, and morphology of the samples were also investigated. The effect of GNPs addition on the electrical conductivity of hydrogel was assessed. The cytotoxicity of hydrogels was assessed through MTT assay in the exposure of H9C2 cells up to 7 days. Scanning electron microscopy was applied to evaluate the morphology of seeded cells. The synthesis parameters of PGS nanoparticles were optimized through which 2.5%w/v of PGS and 1:10 organic phase to aqueous phase (O/A) ratio were found desirable. The optimum hydrogel illustrated 2 min gelation time and was stable up to 20 days with 5% swelling in the first 12 h into phosphate buffered saline. The GNPs with a uniform distribution rendered the hydrogel electrically conductive (1500 μS/cm). According to the MTT assay results, 3.125 μM melatonin was considered as the suitable concentration by which a significant increase in the cell viability was observed. The results exhibited that the prepared hydrogel composed of pluronic/chitosan/GNPs, and 3.125 μM melatonin-loaded PGS nanoparticles could be applied as a promising scaffold for myocardial tissue engineering. © 2020 Wiley Periodicals LLC.
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