The Effect of Aqueous Extract of Rosa Damascena on Formaldehyde-Induced Toxicity in Mice Testes Publisher Pubmed



Askaripour M¹ ; Hasanpour A² ; Hosseini F³ ; Moshrefi M⁴ ; Moshtaghi G⁵ ; Hasannejad M⁶ ; Rajabi S¹ ; Nematollahimahani SN⁷ Show Affiliations
Source: Pharmaceutical Biology Published:2018

Abstract

Context: Rosa damascena L. (Rosaceae) (RD) essential oil and extracts are commonly used as a flavour in herbal medicine which increase libido. Previous studies have shown inhalation of RD flower’s oil increases libido and causes protective effects in formaldehyde (FA)-induced testicular damage. Objective: The protective effects of aqueous extract of RD on the male reproductive system of mice were examined following FA-induced damage. Materials and methods: Forty-eight adult NMRI male mice were randomly assigned to six groups (n = 8): control (normal saline, 10 mg/kg); RD40 (40 mg/kg, p.o.); FA treated (10 mg/kg of 10%, i.p.) and FA+D treated at 10, 20 and 40 mg/kg (FA+D10), (FA+D20) and (FA+D40), respectively, for 40 days. At the end of treatment regimes, serum testosterone (T) level and the reproductive activity, viz. body/organ weights, testicular structure and sperm characteristics were studied. Results: Formaldehyde administration significantly decreased serum T level (p<0.001), testicular weight/volume, tubular diameter and sperm characteristics compared to the control group (p<0.05). RD (40mg/kg) administration in FA-treated mice significantly improved serum T level, testicular weight/histological structure, tubular diameter, Leydig cell number and epididymal sperm characteristics in comparison to its lower doses and the control group (p<0.05). Discussion and conclusions: We may conclude that RD flower extract can withstand effects of FA in the male reproductive system of mice possibly due to its antioxidative properties. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.