Novel Chitosan-Quinic Acid Nanoparticles Labeled With M99 Technetium for Breast Cancer Imaging Publisher



Zia A¹ ; Aghdam HR² ; Saffari M¹ ; Farzaneh J² ; Hamedani MP³ ; Ebrahimi SES⁴ ; Abdollahi K² ; Kheirabad SS² ; Zarringhalam F² ; Omoomi FD² ; Farhoudi R⁴ ; Ardestani MS² Show Affiliations
Source: Nanomedicine Journal Published:2022

Abstract

Objective(s): Today, cancer is one of the health concerns in modern societies. The use of nanoparticles in medical science has created new possibilities for diagnosis, imaging of tumors, and treatment of cancer in humans. Materials and Methods: In this study, chitosan nanoparticles modified with quinic acid were used to diagnose breast cancer using a single-photon emission computed tomography imaging technique. For this purpose, quinic acid was activated by EDC/NHS and then binding was performed by adding chitosan nanoparticles. Results: TEM, DLS, FTIR, and LC/MASS analyzes were used to investigate this conjugation. MTT toxicity test showed no toxicity on the HEK-293 cell lines and therapeutic properties for the MCF-7 cell line. The response surface method depends on the central composite design that was applied. For the highest labeling efficiency, using 3 factors: the amount of chitosan-quinic acid, pH, and SnCl2 as a reducing agent, and the efficiency of labeling was evaluated by thin-layer chromatography (TLC). Conclusion: Finally, the study of biodistribution using SPECT imaging in cancer mice and its comparison with blocking tests showed that this compound has a good ability to diagnose breast cancer. © 2022 Nanomedicine Journal. All rights reserved.