Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production

Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production Publisher Pubmed

Abdoli A^{1, 2} ; Soleimanjahi H¹ ; Jamali A³ ; Mehrbod P³ ; Gholami S³ ; Kianmehr Z⁴ ; Feizi N³ ; Saleh M³ ; Bahrami F⁵ ; Mokhtariazad T⁶ ; Abdoli M³ ; Kheiri MT³

Show Affiliations

1. Departments of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2. Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
3. Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran
4. Immunoregulation Research Center, Shahed University, Tehran, Iran
5. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
6. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Biotechnology Letters Published:2016

Abstract

Objectives: To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection. Results: Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however, H3N2 virus grown in MDCK-SIAT1 had lower viral titers in comparison with MDCK cells. The optimized MOIs to infect the cells on plates and microcarrier were selected 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively. Conclusions: MDCK-SIAT1 cells may be considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic stability and high titer of influenza virus production. © 2016, Springer Science+Business Media Dordrecht.

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Style	Citing Format
MLA	Abdoli A, et al.. "Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production." Biotechnology Letters, vol. 38, no. 6, 2016, pp. 941-948.
APA	Abdoli A, Soleimanjahi H, Jamali A, Mehrbod P, Gholami S, Kianmehr Z, Feizi N, Saleh M, Bahrami F, Mokhtariazad T, Abdoli M, Kheiri MT (2016). Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production. Biotechnology Letters, 38(6), 941-948.
Chicago	Abdoli A, Soleimanjahi H, Jamali A, Mehrbod P, Gholami S, Kianmehr Z, Feizi N, et al.. "Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production." Biotechnology Letters 38, no. 6 (2016): 941-948.
Harvard	Abdoli A et al. (2016) 'Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production', Biotechnology Letters, 38(6), pp. 941-948.
Vancouver	Abdoli A, Soleimanjahi H, Jamali A, Mehrbod P, Gholami S, Kianmehr Z, et al.. Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production. Biotechnology Letters. 2016;38(6):941-948.
BibTex	@article{ author = {Abdoli A and Soleimanjahi H and Jamali A and Mehrbod P and Gholami S and Kianmehr Z and Feizi N and Saleh M and Bahrami F and Mokhtariazad T and Abdoli M and Kheiri MT}, title = {Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production}, journal = {Biotechnology Letters}, volume = {38}, number = {6}, pages = {941-948}, year = {2016} }
RIS	TY - JOUR AU - Abdoli A AU - Soleimanjahi H AU - Jamali A AU - Mehrbod P AU - Gholami S AU - Kianmehr Z AU - Feizi N AU - Saleh M AU - Bahrami F AU - Mokhtariazad T AU - Abdoli M AU - Kheiri MT TI - Comparison Between Mdck and Mdck-Siat1 Cell Lines As Preferred Host for Cell Culture-Based Influenza Vaccine Production JO - Biotechnology Letters VL - 38 IS - 6 SP - 941 EP - 948 PY - 2016 ER -

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