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Intrafamilial Transmission of Helicobacter Pylori: Genotyping of Faecal Samples Publisher Pubmed



Mamishi S1, 2 ; Eshaghi H1 ; Mahmoudi S2 ; Bahador A3 ; Hosseinpour Sadeghi R2 ; Najafi M4 ; Farahmand F4 ; Khodadad A4 ; Pourakbari B2
Authors
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Authors Affiliations
  1. 1. Department of Pediatric Infectious Disease, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Pediatric Infectious Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pediatric Gastroenterology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: British Journal of Biomedical Science Published:2016


Abstract

Background: After more than 20 years of research, there is a little information about the detailed routes of Helicobacter pylori transmission. The aim of this study was to explore intrafamilial transmission of H. pylori in children who had indication for upper gastrointestinal endoscopy and their parents. Methods: Children (aged up to 15 years) were studied during September 2012 to October 2013. The parents of those with positive urea breath test results were asked to provide faecal and blood samples after informed consent. Non-invasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in faeces were measured. The genetic similarity of the family strains was investigated by the random amplification of polymorphic DNA (RAPD-PCR) genotyping method. Results: According to the genotyping results of 30 families, in 10 (33.3%) children related H. pylori genotypes to their mothers were found, while only 2 children (6.7%) had similar genotypes to their fathers. Interestingly, children with similar H. pylori genotype with their mothers had higher IgA (35.7 ± 10.8) and IgM antibody titres (87.23 ± 19.15) than other children. In addition, in these children, lower titres of IgG antibodies (9.93 ± 3.31) were found rather than children who had no H. pylori in their faeces or had no similarities with their parents (30.28 ± 6.15). Conclusions: In conclusion, mother-to-child transmission is the main route of intrafamilial transmission of H. pylori in Iranian families. Molecular typing of H. pylori can be useful in identifying a high-risk population. © 2016 British Journal of Biomedical Science.
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