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Melatonin Affects Membrane Integrity, Intracellular Reactive Oxygen Species, Caspase3 Activity and Akt Phosphorylation in Frozen Thawed Human Sperm Publisher Pubmed



Najafi A1 ; Adutwum E2 ; Yari A3 ; Salehi E1 ; Mikaeili S1 ; Dashtestani F4 ; Abolhassani F1 ; Rashki L5 ; Shiasi S1 ; Asadi E1
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Science, Building number 2, 16 Azar Street, Keshavarz Street, Tehran, 14155-6447, Iran
  2. 2. Department of Medicine, School of Medicine, Tehran University of Medical Science, Tehran, Iran
  3. 3. Department of Anatomical Sciences, School of Medicine, Alborz University of Medical Science, Alborz, Iran
  4. 4. Institute of Biochemistry and Biophysics, Tehran University, Tehran, Iran
  5. 5. Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute, Tehran, Iran

Source: Cell and Tissue Research Published:2018


Abstract

Cryopreservation is known to induce oxidative stress in spermatozoa. Although melatonin has powerful antioxidant properties, little is known about its effects on human sperm quality during cryopreservation. The present study was undertaken to investigate the effects of melatonin treatment on human sperm parameters essential for fertilization. We first evaluated the effects of various concentrations of melatonin (0–15 mM) on human sperm parameters such as motility, viability and levels of intracellular reactive oxygen species during cryopreservation in order to identify an optimal dose with the greatest effects for further studies. Liquefied semen samples were then divided into three aliquots: cryopreserved without melatonin (control), cryopreserved with 3 mM melatonin and fresh groups. After being thawed, samples were evaluated for motility, viability, membrane integrity, intracellular reactive oxygen species levels, caspase-3 activity and AKT phosphorylation. Treatment of spermatozoa with the various concentrations of melatonin significantly increased their motility and viability and decreased their intracellular reactive oxygen species levels compared with the control group. The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group. Thus, melatonin exerts protective effects against cryodamage during human spermatozoa cryopreservation and may exert its effects via the PI3K/AKT signaling pathway. © 2017, Springer-Verlag GmbH Germany, part of Springer Nature.
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