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The Profile of Toll-Like Receptor 2 (Tlr2), Tlr4 and Their Cytosolic Downstream Signaling Pathway in Common Variable Immunodeficiency (Cvid) Patients Pubmed



Sharifi L1, 2 ; Aghamohammadi A1 ; Rezaei N1, 3, 4 ; Yazdani R1 ; Mahmoudi M5 ; Amiri MM6 ; Masoumi F4 ; Bokaie S7 ; Tavasolian P4 ; Sanaei R8 ; Moshiri M9 ; Tavakolinia N1 ; Alinia T1 ; Azizi G10 Show All Authors
Authors
  1. Sharifi L1, 2
  2. Aghamohammadi A1
  3. Rezaei N1, 3, 4
  4. Yazdani R1
  5. Mahmoudi M5
  6. Amiri MM6
  7. Masoumi F4
  8. Bokaie S7
  9. Tavasolian P4
  10. Sanaei R8
  11. Moshiri M9
  12. Tavakolinia N1
  13. Alinia T1
  14. Azizi G10
  15. Mirshafiey A1, 6
Show Affiliations
Authors Affiliations
  1. 1. Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Sheffield, United Kingdom
  4. 4. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  8. 8. Department of Immunology, Iran University of Medical Sciences, Tehran, Iran
  9. 9. Department of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  10. 10. Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran

Source: Iranian Journal of Allergy# Asthma and Immunology Published:2018


Abstract

Common variable immunodeficiency (CVID) is the most common clinical primary antibody deficiency, characterized by increased susceptibility to recurrent bacterial infections. Since Toll-like receptors (TLRs) play an important role in the maturation and differentiation of B-cells, TLRs’ defect can be involved in the pathogenesis of CVID. Therefore, we evaluated the expression of TLR2 and TLR4 and their signaling pathway; also their association with autoimmunity, B-cell subtypes and response to pneumovax-23 were assessed in CVID patients. Sixteen CVID patients were enrolled in the study. Flow cytometry was used for assessing the protein expression of TLR2 and TLR4, and real-time PCR was used for gene expression of myeloid differentiation primary response 88 (MyD88) and toll interacting protein (Tollip). We found a higher protein expression of TLR2 in CVID patients which was associated with lower number of end stage B-cells and hyporesponse to pneumovax-23 vaccination. We showed a lower mRNA expression of MyD88 and an almost equal Tollip mRNA expression in CVID patients compared with controls. There was a profound association between MyD88 gene expression and autoimmunity in CVID patients. According to the presence of the lower number of end stage B-cells and poor vaccine response in CVID patients and their correlation with the higher expression of TLR2, we hypothesized that there is a functional defect in this receptor and/or its downstream in the peripheral blood mononuclear cells (PBMCs) of CVID patients. Copyright© April 2018, Iran J Allergy Asthma Immunol. All rights reserved.
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