Biomarkers for Oral Squamous Cell Carcinoma (Mir-24, Mir-200, and Mir-34): Screening and Detection Microrna Publisher Pubmed



N Bahrami NAGHMEH ; M Pirrafiee MARYAM ; F Azadi FATEMEH ; R Azimnejad REZVAN ; Szf Kiaei Seyedeh Zahra FOTOOK ; Aj Abbasi Amir JALAL ; Mk Kazempourdizaji Mehdi KAZEMPOOR ; S Lookzadeh SOMAYEH ; Smr Nejatollahi Seyed Mohammad REZA ; M Daustani MAHYA
Source: Asian Pacific Journal of Cancer Prevention Published:2024

Abstract

Introduction: Oral squamous cell carcinoma (OSCC) includes about 90% of all oral malignant tumors, and most of them are diagnosed in advanced stages. This study investigated the expression changes of miR-24, miR-200, and miR-34 in saliva samples of patients with oral squamous cell carcinoma, for early diagnosis. Methods: In this study, 30 patients and 30 healthy individuals were selected. After RNA extraction and cDNA synthesis, the expression levels of miR-24, miR-200, and miR-34 in saliva samples were measured and evaluated using the Real-Time PCR technique. Results: Folding change calculation using 2^(-∆∆ Ct) refers to the relative difference in the expression of the markers of the two groups. The expression level of two biomarkers, miR-200 and miR-34, is decreased in patients compared to healthy people; and the expression level of miR-24 is increased in patients compared to healthy people. Conclusion: In general, considering the availability and convenience of saliva sample collection for early detection of the disease, this research result can be considered a diagnostic screening test. To further prove the research results, conducting more extensive studies with more samples is recommended. © 2024 Elsevier B.V., All rights reserved.