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Development of Ultrasound-Assisted Dispersive Liquid–Liquid Microextraction Based on in Situ Magnetic Deep Eutectic Solvent for Determination of Cefixime in Biological Samples by High-Performance Liquid Chromatography Publisher



Abdi K1, 2 ; Ezoddin M3 ; Behnamipour S4 ; Taghizadeh T3
Authors
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Authors Affiliations
  1. 1. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Iranian National Center for Addiction Studies (INCAS), Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Chemistry, Payame Noor University(PNU), Tehran, Iran
  4. 4. Research Center of Environmental Pollutants, Qom University of Medical Sciences, Qom, Iran

Source: Journal of the Iranian Chemical Society Published:2024


Abstract

In this study, ultrasound-assisted dispersive liquid–liquid microextraction based on in situ magnetic deep eutectic solvent (UA-DLLME-IM-DES) was considered for the determination of cefixime drug in the biological samples by high-performance liquid chromatography. A deep eutectic solvent magnetized (MDES), [tetrabutylammonium bromide/decanoic acid][FeCl3], was prepared through an in situ combination of the deep eutectic solvent (DES) and FeCl3 in the sample solution. Ultrasonication was performed to increase the generation speed of the magnetic DES in the solution and enhance the mass transfer of the analyte into the magnetic deep eutectic solvent. The characterization of the synthesized component was performed by Fourier transform infrared (FTIR) and vibrating sample magnetometry (VSM). Some factors influencing the extraction yield were assessed. Based on following the optimization, the calibration curve for the analyte was in the ranges of 2–1000 µg L−1, with a correlation coefficient higher than 0.9954. The limits of detection (LODs) and limits of quantification (LOQs) were 0.5 and 1.6 µg L−1 in real samples, respectively. The intra-day and inter-day precision was in the range of 33–3.9% and 4.5–5.2%, respectively. The extraction recoveries and enrichment factors of the analyte were acquired to be in the range of 92.1–93.8% and 125, respectively. Ultimately, the method was handled to extract cefixime drug in wastewater, human urine, and plasma samples. © Iranian Chemical Society 2024.