High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica

High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica Publisher

Hosseinisafa A¹ ; Rokni MB¹ ; Mosawi SH² ; Heydarian P³ ; Azizi H⁴ ; Davari A¹ ; Aryaiepour M^{1, 5}

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1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Biology and Microbiology, Faculty of Medical Technology, Khatam Al-Nabieen University, Kabul, Afghanistan
3. Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
4. Department of Microbiology and Parasitology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
5. Department of Medical Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Public Health Published:2019

Abstract

Background: Fasciolosis is a shared disease between humans and livestock caused by hepatic trematodes; Fasciola hepatica and F. gigantica. Differentiate between the two species of this genus is essential. High-Resolution Melting (HRM) Analysis represents a new approach to this issue. This method can be performed right after termination of Real-Time PCR. This technique has not been used for identification of adult F. hepatica and F. gigantica genotypes. The aim of this study was to determine Fasciola species by using HRM in isolates taken from Iran, respectively. Methods: Ninety-three Fasciola spp. samples were collected from infected slaughtered animals in different regions of Iran, including North West (Ardebil Province) and South East (Zahedan Province) during 2016. Genomic DNA from the samples was extracted using a DNA extraction kit and then after Real-Time PCR amplification, HRM was done. Results: Overall, 59 and 34 isolates were identified as F. hepatica and F. gigantica, respectively. The percentages of each species from animals were as follows: sheep (F. hepatica, 80.39% and F. gigantica, 19.61%), cattle (F. hepatica, 42.85% and F. gigantica, 57.15%). Conclusion: HRM technique developed in the present study is a powerful, rapid and sensitive technique for epidemiological survey and molecular identification between F. hepatica and F. gigantica. © 2019, Iranian Journal of Public Health. All rights reserved.

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Style	Citing Format
MLA	Hosseinisafa A, et al.. "High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica." Iranian Journal of Public Health, vol. 48, no. 3, 2019, pp. 501-507.
APA	Hosseinisafa A, Rokni MB, Mosawi SH, Heydarian P, Azizi H, Davari A, Aryaiepour M (2019). High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica. Iranian Journal of Public Health, 48(3), 501-507.
Chicago	Hosseinisafa A, Rokni MB, Mosawi SH, Heydarian P, Azizi H, Davari A, Aryaiepour M. "High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica." Iranian Journal of Public Health 48, no. 3 (2019): 501-507.
Harvard	Hosseinisafa A et al. (2019) 'High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica', Iranian Journal of Public Health, 48(3), pp. 501-507.
Vancouver	Hosseinisafa A, Rokni MB, Mosawi SH, Heydarian P, Azizi H, Davari A, et al.. High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica. Iranian Journal of Public Health. 2019;48(3):501-507.
BibTex	@article{ author = {Hosseinisafa A and Rokni MB and Mosawi SH and Heydarian P and Azizi H and Davari A and Aryaiepour M}, title = {High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica}, journal = {Iranian Journal of Public Health}, volume = {48}, number = {3}, pages = {501-507}, year = {2019} }
RIS	TY - JOUR AU - Hosseinisafa A AU - Rokni MB AU - Mosawi SH AU - Heydarian P AU - Azizi H AU - Davari A AU - Aryaiepour M TI - High-Resolution Melting Analysis As an Appropriate Method to Differentiate Between Fasciola Hepatica and F. Gigantica JO - Iranian Journal of Public Health VL - 48 IS - 3 SP - 501 EP - 507 PY - 2019 ER -

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