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Effect of Photobiomodulation on Structure and Function of Extracellular Vesicle Secreted From Mesenchymal Stem Cells Publisher Pubmed



Gholami L1, 2 ; Khorsandi K3, 4 ; Nooshabadi VT5 ; Shahabi S6 ; Jazaeri M7 ; Esfahani H3 ; Faradonbeh DR8 ; Malekshahi ZV8 ; Afsartala Z9 ; Mostafa N10
Authors
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Authors Affiliations
  1. 1. Department of Periodontics, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada
  3. 3. Department of Photodynamic, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
  4. 4. Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC, United States
  5. 5. Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, Semnan University of Medical Science, Semnan, Iran
  6. 6. Dental Implants Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  7. 7. Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  8. 8. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, Iran
  10. 10. Department of Oral Health Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada

Source: Photochemistry and Photobiology Published:2022


Abstract

The current study intended to evaluate the effect of photobiomodulation on the morphology and function of EVs secreted from mesenchymal stem cells (MSCs) derived from periodontal ligament (PDL) and the adipose tissue (ADSCs) (from buccal fat pad) in vitro. These cells were irradiated at 660 nm or kept in dark as control. EVs were then isolated from each group using ultracentrifugation. EVs were defined by flow cytometry and Western blot. Electron microscopy (SEM) was used to study the morphology of EVs. Then, MTT and wound-healing scratch assays were applied to compare the cell survival and migration of human dermal fibroblast (HDF) cells treated with the EVs obtained from the four groups. According to SEM images, isolated EV were round and cup-shaped in all groups showing no destructive effects of laser irradiation on EV morphology. MTT test results revealed a statistically significant difference between the HDF cells treated with different EV groups from hPDLSCs-Dark in comparison with control (0 μg/mL) (P < 0.05) and treated with exosome from hPDLSCs-Irradiation cells compared with dark group (P < 0.05). However, scratch wound-healing assay did not show a significant difference between various groups (P ˃ 0.05). Further studies with different irradiation protocols are recommended to find an optimal strategy. © 2022 The American Society for Photobiology.
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