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Expression of Neutrophil Gelatinase-Associated Lipocalin (Ngal) in Peripheral Nerve Repair Publisher Pubmed



Aghanasir F1 ; Aghaei H2 ; Imani Fooladi AA3 ; Ebrahimi M4 ; Bagherpour G5 ; Nourani MR6, 7
Authors
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Authors Affiliations
  1. 1. Department of Physiology and Biophysics, Baqiyatallah University of Medical Sciences, Tehran, Iran
  2. 2. Department of Physiology, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  4. 4. Organ Anatomy Department, Graduate School of Medicine, Tohoku University, Sendai, Japan
  5. 5. Department of Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran
  6. 6. Tissue Engineering Division, Baqiyatallah University of Medical Sciences, Tehran, Iran
  7. 7. Systems Biology Institute, Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: Journal of Receptors and Signal Transduction Published:2016


Abstract

Trauma is one of the causes of peripheral nerve injuries. Free radicals increase after tissue damage. Free radicals are usually scavenged and detoxied by antioxidants. In this study, we assessed the antioxidative role of the NGAL molecule in sciatic nerve repair in rats. Materials and methods: The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the NGAL gene was confirmed by RT-PCR. For immunohistochemistry analysis, the samples were xed in paraformaldehyde and cut in 20 micrometer slices by cryostat. Results: The expression of NGAL signicantly upregulated in day 1, 3 and week 1 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also confirmed the protein expression of this gene. Discussion: The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair. © 2016 Informa UK Limited, trading as Taylor and Francis Group.
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