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Sensitive Colorimetric Aptasensor Based on G-C3n4@Cu2o Composites for Detection of Salmonella Typhimurium in Food and Water Publisher Pubmed



Tarokh A1 ; Pebdeni AB1 ; Othman HO2 ; Salehnia F3 ; Hosseini M1, 4
Authors
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Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
  2. 2. Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil, Iraq
  3. 3. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, 1417614418, Iran
  4. 4. Department of Pharmaceutical Biomaterials, Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Microchimica Acta Published:2021


Abstract

A new colorimetric aptasensor equipped with a novel composite of graphitic carbon nitride (g-C3N4) nanosheets and copper oxide(I) (Cu2O) nanocrystals is presented for Salmonella typhimurium (S.typhimurium). The dual-purpose structure of this composite simultaneously contributes to superb peroxidase-like activity and interaction with a label-free aptamer. Although g-C3N4@Cu2O effectively creates a visible blue color following the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in presence of hydrogen peroxide (H2O2), this catalytic activity of the composite severely decreases after the interaction with aptamers. In the presence of S. typhimurium in sample, aptamers bound to their specific target. Subsequently, g-C3N4@Cu2O catalytic activity was enhanced in proportion to S. typhimurium concentration. Under optimized conditions, this aptasensor exhibited an excellent detection performance in a range from 1.5 × 101 to 1.5 × 105 CFU/ml, with a detection limit of 15 CFU/ml. Besides, portable detection of S. typhimurium by paper-based model of this method operated successfully in just 6 min. Analysis of spiked milk samples revealed high potential of this method as a sensitive, rapid, and label-free promising tool for S. typhimurium detection. Graphical abstract: [Figure not available: see fulltext.]. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, AT part of Springer Nature.
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