Standard Operating Procedure for Production of Mouse Brown Adipose Tissue-Derived Mesenchymal Stem Cells Publisher Pubmed



Arjmand B¹ ; Rezaeitavirani M² ; Alavimoghadam S¹ ; Tayanloobeik A¹ ; Gholami M³ ; Kokabihamidpour S¹ ; Arjmand R¹ ; Rezazadehmafi A⁵ ; Mohamadijahani F⁶ ; Larijani B⁷
Source: Methods in Molecular Biology Published:2024

Abstract

Over the past years, stem cell technology was heralded as a significant breakthrough of the century in scrutinizing the intricacies of human body biology and discovering different therapeutic approaches. Recently, adipose tissue, as a suitable source of harvesting mesenchymal stem cells, has attracted the attention of many researchers in the field of regenerative medicine. Adipose tissue-derived mesenchymal stem cells can self-renew and differentiate into different types of cells such as adipocytes, chondrocytes, and osteoblasts. Adipose tissue, especially brown type, is considered an attractive cell source for various therapeutic purposes, such as restoring damaged tissue or fighting against diseases such as obesity. The growth of importance of stem cell applications in regenerative medicine has highlighted the need to seek appropriate mesenchymal stem cells sources. Recently, in the light of many efforts in the field of regenerative medicine, mice have gained increasing interest as a suitable source of adipose tissue for the extraction of mesenchymal stem cells, which can be used in the preclinical investigations in order to aid in the treatment of many human diseases. © Springer Science+Business Media, LLC 2023.