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The Effects of All Trans Retinoic Acid, Vitamin D3 and Their Combination on Plasma Levels of Mirna-125A-5P, Mirna-34A, and Mirna-126 in an Experimental Model of Diabetes Publisher



Sharifzadeh M1 ; Esmaeilibandboni A2, 3 ; Emami MR1 ; Naeini F1 ; Zarezadeh M4, 5 ; Javanbakht MH6
Authors
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Authors Affiliations
  1. 1. Department of Clinical Nutrition, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences (TUMS), Tehran, Iran
  2. 2. Department of Medical Genetics, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  3. 3. Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  4. 4. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  5. 5. Nutrition Research Center, Department of Clinical Nutrition, School of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences (TUMS), Tehran, Iran

Source: Avicenna Journal of Phytomedicine Published:2022


Abstract

Objective: The purpose of this study was to evaluate the effects of ATRA (all trans retinoic acid), vitamin D3, and their combination on circulating levels of miR (MicroRNA) -125a-5p, miR-126, and miR-34ain diabetic rats. Materials and Methods: Total miRNA was extracted from plasma samples. miRNA expression profiles of 30 rats in five groups were analyzed after 4-week intervention. The expression levels of miRNAs were measured using qRT-PCR. Results: We analyzed the expression of miR-126, miR-125a-5p, and miR-34a in serum among all five groups (p=0.268). The levels of miRNA-126 (p=0.004) and miR-125a-5p (p=0.014) showed a significant difference among our experimental groups. The circulating levels of miR-126 decreased in DC (Diabetic control) group compared to the HC (Healthy control) group (p=0.009). In addition, vitamin D3+ATRA supplementation increased miR-126 expression (p=0.014). Moreover, the levels of miR-125a-5p decreased in the DC group compared to the HC group (p=0.019). Conclusion: The expression of miR-126 and miR-125a-5p decreased in diabetic rats. Also, vitamin D3+ATRA can be considered a new therapeutic agent that can elevate miR-126 expression and prevent diabetes-related cardiovascular complications. © 2022 Mashhad University of Medical Sciences. All rights reserved.
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