Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells

Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells Publisher Pubmed

Mobarra N¹ ; Soleimani M² ; Pakzad R^{3, 4} ; Enderami SE⁵ ; Pasalar P⁶

Show Affiliations

1. Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2. Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3. Department of Epidemiology, Faculty of Health, Ilam University of Medical Sciences, Ilam, Iran
4. Departments of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5. Stem Cell Technology Research Center, Tehran, Iran
6. Metabolic disorder Research center, Endocrinology and Metabolism, Molecular sciences institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2018

Abstract

Nanofibrous scaffolds are considered as a new strategy for Type 1 diabetes mellitus therapy. We used a hybrid of poly-l-lactic acid (PLLA) and polycaprolactone (PCL) as three-dimensional (3D) culture models for differentiation of human induced pluripotent stem cells (hiPSCs) to beta islet-like cluster cell compared with routine culture (2D). Morphological changes of cells were checked by microscope. mRNA endodermal SOX-17 on day 7 and pancreatic gene markers Pdx1, glucagon and Glut2 were evaluated on day 23 by qPCR. As well as, insulin and C-peptide protein expression was evaluated by immunocytochemistry staining. In addition, insulin and C-peptide secretion in various glucose concentrations was evaluated by ELISA. Light and scanning electron microscopy (SEM) microscope showed changes in induced cells. In tandem, these modifications were more evident in 3 D culture. Pdx1, Glucagon and Glut2 markers in PLLA/PCL were significantly higher in 3 D culture. In addition, qualitative immunochemistry showed that insulin and C-peptide were expressed in 2 D and 3 D culture medium. Furthermore, evaluation of insulin and C-peptide clarified that secretion of these proteins in PLLA/PCL scaffold were statistically different in 2 D and 3 D strategies. These findings suggest that functional matured induction cells on PLLA/PCL scaffold can be used for islet beta cell therapy and regenerative medicine. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

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Style	Citing Format
MLA	Mobarra N, et al.. "Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells." Artificial Cells# Nanomedicine and Biotechnology, vol. 46, no. sup3, 2018, pp. S685-S692.
APA	Mobarra N, Soleimani M, Pakzad R, Enderami SE, Pasalar P (2018). Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells. Artificial Cells# Nanomedicine and Biotechnology, 46(sup3), S685-S692.
Chicago	Mobarra N, Soleimani M, Pakzad R, Enderami SE, Pasalar P. "Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells." Artificial Cells# Nanomedicine and Biotechnology 46, no. sup3 (2018): S685-S692.
Harvard	Mobarra N et al. (2018) 'Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells', Artificial Cells# Nanomedicine and Biotechnology, 46(sup3), pp. S685-S692.
Vancouver	Mobarra N, Soleimani M, Pakzad R, Enderami SE, Pasalar P. Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells. Artificial Cells# Nanomedicine and Biotechnology. 2018;46(sup3):S685-S692.
BibTex	@article{ author = {Mobarra N and Soleimani M and Pakzad R and Enderami SE and Pasalar P}, title = {Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells}, journal = {Artificial Cells# Nanomedicine and Biotechnology}, volume = {46}, number = {sup3}, pages = {S685-S692}, year = {2018} }
RIS	TY - JOUR AU - Mobarra N AU - Soleimani M AU - Pakzad R AU - Enderami SE AU - Pasalar P TI - Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells JO - Artificial Cells# Nanomedicine and Biotechnology VL - 46 IS - sup3 SP - S685 EP - S692 PY - 2018 ER -

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