Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Bladder Smooth Muscle Cell Differentiation of the Human Induced Pluripotent Stem Cells on Electrospun Poly(Lactide-Co-Glycolide) Nanofibrous Structure Publisher Pubmed



Mirzaei A1, 2 ; Saburi E3 ; Islami M4 ; Ardeshirylajimi A5 ; Omrani MD6 ; Taheri M6 ; Moghadam AS7 ; Ghafourifard S8
Authors
Show Affiliations
Authors Affiliations
  1. 1. Cellular & Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
  2. 2. Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
  3. 3. Immunogenetics and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  4. 4. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  5. 5. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  7. 7. Bu-Ali Research Institute, Department of Immunogenetics, Mashhad University of Medical Sciences, Mashhad, Iran
  8. 8. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Gene Published:2019


Abstract

Smooth muscle cell (SMC) regeneration plays an important role in retrieving the bladder-wall functionality and it can be achieved by a proper cell-co-polymer constructed by tissue engineering. Human induced pluripotent stem cells (iPSCs), which can be specifically prepared for the patient, was considered as cells in this study, and Poly(lactide-co-glycolide) (PLGA) as a most interesting polymer in biomedical applications was applied to the scaffold fabrication by electrospinning. After scaffold characterization, SMC differentiation potential of the human iPSCs was investigated while cultured on the PLGA nanofibrous scaffold by evaluation of the SMC related important gene and protein markers. Alpha-smooth muscle actin (ASMA), Smooth muscle 22 alpha (SM-22a) as two early SMC markers were significantly up regulated either two and three weeks after differentiation induction in human iPSCs cultured on PLGA compared to those cells cultured on the tissue culture polystyrene (TCPS). But Calponin-1, Caldesmon1 and myosin heavy chain (MHC) expression differences in human iPSCs cultured on PLGA and TCPS were significant only three weeks after differentiation induction based on its lately expression in the differentiation process. ASMA and MHC proteins were also considered for evaluation by immunocytochemistry on differentiated iPSCs whereas results showed higher expression of these proteins in stem cells grown on PLGA compared to the TCPS. According to the results, human iPSCs demonstrated a great SMC differentiation potential when grown on PLGA and it could be considered as a promising cell-co-polymer for use in bladder tissue engineering. © 2019 Elsevier B.V.
Related Docs
View other Related Docs
1. Poly (3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Improved Osteogenic Differentiation of the Human Induced Pluripotent Stem Cells While Considered As an Artificial Extracellular Matrix, Journal of Cellular Physiology (2019)
2. In Vitro Osteogenic Differentiation Potential of the Human Induced Pluripotent Stem Cells Augments When Grown on Graphene Oxide-Modified Nanofibers, Gene (2019)
3. Comparison of Osteogenic Differentiation Potential of Induced Pluripotent Stem Cells on 2D and 3D Polyvinylidene Fluoride Scaffolds, Journal of Cellular Physiology (2019)
4. Incorporated-Bfgf Polycaprolactone/Polyvinylidene Fluoride Nanocomposite Scaffold Promotes Human Induced Pluripotent Stem Cells Osteogenic Differentiation, Journal of Cellular Biochemistry (2019)
5. Applied Induced Pluripotent Stem Cells in Combination With Biomaterials in Bone Tissue Engineering, Journal of Cellular Biochemistry (2017)
6. Bone Tissue Engineering: Adult Stem Cells in Combination With Electrospun Nanofibrous Scaffolds, Journal of Cellular Physiology (2018)
7. Improved Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Cultured on Polyvinylidene Fluoride/Collagen/Platelet-Rich Plasma Composite Nanofibers, Journal of Cellular Physiology (2020)
8. Improvement of Hepatogenic Differentiation of Ips Cells on an Aligned Polyethersulfone Compared to Random Nanofibers, Artificial Cells# Nanomedicine and Biotechnology (2018)
Experts (# of related papers)
View all Related Experts
Seyed Mohammad Kazem Aghamir Mohammad Ali (2)
Rashin Mohseni (1)
Other Related Docs
9. Collagen Coated Electrospun Polyethersulfon Nanofibers Improved Insulin Producing Cells Differentiation Potential of Human Induced Pluripotent Stem Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
10. Comparison of Human-Induced Pluripotent Stem Cells and Mesenchymal Stem Cell Differentiation Potential to Insulin Producing Cells in 2D and 3D Culture Systems in Vitro, Journal of Cellular Physiology (2020)
11. Induced Pluripotent Stem Cells As a New Getaway for Bone Tissue Engineering: A Systematic Review, Cell Proliferation (2017)
12. Pcl/Pva Nanofibrous Scaffold Improve Insulin-Producing Cells Generation From Human Induced Pluripotent Stem Cells, Gene (2018)
13. Biomimetic Scaffold Containing Pvdf Nanofibers With Sustained Tgf-Β Release in Combination With At-Mscs for Bladder Tissue Engineering, Gene (2018)
14. Polyvinyl Alcohol Modified Polyvinylidene Fluoride-Graphene Oxide Scaffold Promotes Osteogenic Differentiation Potential of Human Induced Pluripotent Stem Cells, Journal of Cellular Biochemistry (2020)
15. Microrna-2861 and Nanofibrous Scaffold Synergistically Promote Human Induced Pluripotent Stem Cells Osteogenic Differentiation, Polymers for Advanced Technologies (2020)
16. Collagen-Graft Mixed Cellulose Esters Membrane Maintains Undifferentiated Morphology and Markers of Potential Pluripotency in Feeder-Free Culture of Induced Pluripotent Stem Cells, Biologicals (2016)
17. Generation of High Yield Insulin-Producing Cells (Ipcs) From Various Sources of Stem Cells, Vitamins and Hormones (2021)
18. Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
19. Decellularized Amniotic Membrane Hydrogel Promotes Mesenchymal Stem Cell Differentiation Into Smooth Muscle Cells, FASEB Journal (2024)
20. The Effect of Nanofibre-Based Polyethersulfone (Pes) Scaffold on the Chondrogenesis of Human Induced Pluripotent Stem Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
21. Induced Pluripotent Stem Cell-Derived Extracellular Vesicles: A Novel Approach for Cell-Free Regenerative Medicine, Journal of Cellular Physiology (2019)
22. Generation of Insulin-Producing Cells From Human Induced Pluripotent Stem Cells on Plla/Pva Nanofiber Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)
23. The Role of Micrornas in the Induction of Pancreatic Differentiation, Current Stem Cell Research and Therapy (2020)
24. Bladder Dysfunction, Stem Cells in Urology (2020)
25. Nanofiber-Based Systems Intended for Diabetes, Journal of Nanobiotechnology (2021)
26. Metal-Based Nanostructures/Plga Nanocomposites: Antimicrobial Activity, Cytotoxicity, and Their Biomedical Applications, ACS Applied Materials and Interfaces (2020)
27. Hepatogenic Differentiation of Human Induced Pluripotent Stem Cells on Collagen-Coated Polyethersulfone Nanofibers, ASAIO Journal (2017)
28. Decellularized Amniotic Membrane Scaffolds Improve Differentiation of Ipscs to Functional Hepatocyte-Like Cells, Journal of Cellular Biochemistry (2020)
29. The Generation of Islet-Like Insulin-Producing Cells From Wharton's Jelly-Derived Mesenchymal Stem Cells on the Pes/Fish Gelatin Scaffold, Regenerative Therapy (2024)
30. Synergistic Effects of Polyaniline and Pulsed Electromagnetic Field to Stem Cells Osteogenic Differentiation on Polyvinylidene Fluoride Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2019)
31. Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)
32. Reprogramming of Endometrial Adult Stromal Cells in the Presence of a Rock Inhibitor, Thiazovivin, Could Obtain More Efficient Ipscs, Cell Biology International (2015)
33. Pancreatic Endoderm-Derived From Diabetic Patient-Specific Induced Pluripotent Stem Cell Generates Glucose-Responsive Insulin-Secreting Cells, Journal of Cellular Physiology (2017)
34. Adipose-Derived Stem Cells-Conditioned Medium Improved Osteogenic Differentiation of Induced Pluripotent Stem Cells When Grown on Polycaprolactone Nanofibers, Journal of Cellular Physiology (2019)
35. Stem Cell Therapies for Congenital Heart Disease, Biomedicine and Pharmacotherapy (2016)
36. Toward in Vitro Production of Platelet From Induced Pluripotent Stem Cells, Stem Cell Reviews and Reports (2022)
37. Promoted Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Using Composited Polycaprolactone/Polyvinyl Alcohol/Carbopol Nanofibrous Scaffold, Journal of Drug Delivery Science and Technology (2022)
38. A Comprehensive Overview on Utilizing Electromagnetic Fields in Bone Regenerative Medicine, Electromagnetic Biology and Medicine (2019)
39. Synergism of Electrospun Nanofibers and Pulsed Electromagnetic Field on Osteogenic Differentiation of Induced Pluripotent Stem Cells, ASAIO Journal (2017)
40. Stem Cell Therapy Potency in Personalizing Severe Covid-19 Treatment, Stem Cell Reviews and Reports (2021)
41. Promoting Osteogenic Differentiation of Human-Induced Pluripotent Stem Cells by Releasing Wnt/Β-Catenin Signaling Activator From the Nanofibers, Journal of Cellular Biochemistry (2019)