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A Laccase Heterogeneous Magnetic Fibrous Silica-Based Biocatalyst for Green and One-Pot Cascade Synthesis of Chromene Derivatives Publisher



Mogharabimanzari M1, 2 ; Ghahremani MH3 ; Sedaghat T1 ; Shayan F1 ; Faramarzi MA1
Authors
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Authors Affiliations
  1. 1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411, Iran
  2. 2. The Institute of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
  3. 3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417614411, Iran

Source: European Journal of Organic Chemistry Published:2019


Abstract

Green cascade approaches, which utilize sustainable and recyclable heterogeneous biocatalysts, can be used in the catalysis of multicomponent organic reactions to synthesize biologically important substances. Three magnetic nanoparticles, iron (II, III) oxide, cobalt ferrite, and nickel ferrite, were prepared by co-precipitation and functionalized with fibrous silica (KCC-1), and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and vibrating sample magnetometry (VSM). Laccase immobilized on CoFe 2 O 4 -KCC-1 was used as a heterogeneous biocatalyst for green and one-pot cascade synthesis of 2-amino-5-oxo-4-aryl-4H,5H-pyrano[3,2-c]chromene-3-carbonitriles. The reaction was performed in the presence of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) as a synthetic redox mediator. The optimum reaction conditions were found to be as immobilized laccase (100 mg, 95 U) and TEMPO (2 mol-%) in a 100 mm sodium citrate buffer (pH 4.5), 40 °C, and incubation time 17 h. About 80 % of initial activity of the immobilized laccase was retained after 15 independent runs. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim