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Mesenchymal Stem Cells Pretreatment With Stromal-Derived Factor-1 Alpha Augments Cardiac Function and Angiogenesis in Infarcted Myocardium Publisher Pubmed



Esmaeili R1, 2 ; Darbandiazar A3 ; Sadeghpour A4 ; Majidzadeha K1, 5 ; Eini L6 ; Jafarbeikiravani N1 ; Hoseinpour P7 ; Vajhi A8 ; Oghabi Bakhshaiesh T1 ; Masoudkabir F9 ; Sadeghizadeh M2
Authors
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Authors Affiliations
  1. 1. Genetics Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
  2. 2. Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Rajaie Cardiovascular Medical and Research Centre, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Echocardiography Research Center, Department of Cardiovascular Medicine, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
  6. 6. Cell and Immunotherapy Department, Breast Cancer Research Center, Motamed Cancer Institute ACECR, Tehran, Iran
  7. 7. Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
  8. 8. Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  9. 9. Cardiac Primary Prevention Research Center, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran

Source: American Journal of the Medical Sciences Published:2021


Abstract

Background: Stem cell therapy is among the novel approaches for the treatment of post-myocardial infarction cardiomyopathy. This study aims to compare the effect of stromal-derived factor 1 α (SDF1α), mesenchymal stem cells (MSCs) in combination with the lentiviral production of vascular endothelial growth factor (VEGF) on infarct area, vascularization and eventually cardiac function in a rat model of myocardial infarction (MI). Methods: The influence of SDf1α on MSCs survival was investigated. MSCs were transduced via a lentiviral vector containing VEGF. After that, the effect of mesenchymal stem cell transfection of VEGF-A165 and SDf1α preconditioning on cardiac function and scar size was investigated in five groups of MI rat models. The MSC survival, cardiac function, scar size, angiogenesis, and lymphocyte count were assessed 72 hours and 6 weeks after cell transplantation. Results: SDF1α decreased the lactate dehydrogenase release in MSCs significantly. Also, the number of viable cells in the SDF1α-pretreated group was meaningfully more than the control. The left ventricular systolic function significantly enhanced in groups with p240MSC, SDF1αMSC, and VEGF-A165MSC in comparison to the control group. Conclusions: These findings suggest that SDF1α pretreatment and overexpressing VEGF in MSCs could augment the MSCs' survival in the infarcted myocardium, reduce the scar size, and improve the cardiac systolic function. © 2021 Southern Society for Clinical Investigation
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