Folic Acid Ameliorates Palmitate-Induced Inflammation Through Decreasing Homocysteine and Inhibiting Nf-Κb Pathway in Hepg2 Cells Publisher Pubmed



Bagherieh M¹ ; Kheirollahi A² ; Zamanigarmsiri F¹ ; Emamgholipour S¹ ; Meshkani R¹ Show Affiliations
Source: Archives of Physiology and Biochemistry Published:2023

Abstract

Objective: Prevention of inflammation is one of the possible remedy procedure for steatohepatitis during NAFLD. In this study, we researched the folic acid (FA) potency to attenuate the inflammation of palmitate-treated HepG2 cells and the related signalling pathways. Methods: The molecular mechanisms related to FA anti-inflammatory effect in palmitate and Hcy-treated HepG2 cell line were assessed. Results: The results indicated that while palmitate enhances the expression and secretion of TNF-α, IL-6, and IL-1β, and also intracellular ROS level, FA at concentrations of 25, 50, and 75 µg/mL significantly reversed these effects in HepG2 cells. In addition, FA could ameliorate inflammation and decrease ROS production induced by Hcy. Furthermore, FA pre-treatment suppress palmitate -induced (NF-κB) p65 level in palmitate or Hcy stimulated cells. Conclusions: Overall, these results suggest that FA reduces inflammation in HepG2 cells through decreasing ROS and Hcy concentration level resulting in inhibiting the NF-κB pathway. © 2021 Informa UK Limited, trading as Taylor & Francis Group.