Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme]

Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme] Publisher Pubmed

Salimi A^{1, 2} ; Hamzeloomoghadam M³ ; Irani M⁴ ; Esmaeili S^{4, 5}

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1. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2. Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
3. Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Annales Pharmaceutiques Francaises Published:2025

Abstract

Objectives: World Health Organization (WHO) has reported 249 million cases infected by malaria worldwide and 608 thousands deaths in 2022. Investigations for new antimalarial drugs from traditional medicine have proven to be more effective and less expensive. The medicinal plants that have been used for treatment of malaria, generally known as types of fevers in traditional medicine, can be suitable candidates for evaluating antimalarial effects. The aim of the present study was to evaluate the in vitro mechanism of antimalarial action of selected medicinal plants by inhibition test of heme detoxification (ITHD). Material and methods: The methanol extract and fractions were prepared through maceration of the dry powdered plants. The ITHD method was carried in 96-wells plate and the percentage of heme polymerization inhibition was determined. The cytotoxicity of the effective plants was examined on MDBK cell line by MTT assay. Bioassay guided fractionation was performed exposing to size exclusion column chromatography and liquid-liquid fractionation and was assayed by the ITHD method for the sample with the least IC50 value and lowest cytotoxic effect. Results: The methanol fraction of Viola odorata whole plant showed the most considerable results among the tested plants with IC50 171.8 μg.mL−1 beside the lowest cytotoxic effects. This fraction by the bioassay guided fractionation led to fraction SB2 and this fraction demonstrated the most effective result with lowest IC50 = 14.8 ± 3 μg.mL−1 in ITHD assay. Conclusion: Regarding the results of the present study and the traditional use of V. odorata for overcoming fever in Iranian traditional medicine, the final fraction of the plant could be proper candidate for further phytochemical and antimalarial studies. © 2024 Academie Nationale de Pharmacie

2. Efficacy and Safety of Add-On Viola Odorata L. in the Treatment of Covid-19: A Randomized Double-Blind Controlled Trial, Journal of Ethnopharmacology (2023)

3. Effectiveness of Viola Flower Syrup Compared With Polyethylene Glycol in Children With Functional Constipation: A Randomized, Active-Controlled Clinical Trial, Evidence-based Complementary and Alternative Medicine (2021)

4. The Effect of Viola Odorata Flower Syrup on the Cough of Children With Asthma: A Double-Blind, Randomized Controlled Trial, Journal of Evidence-Based Complementary and Alternative Medicine (2015)

5. Efficacy of Topical Galbanum Oil With Dry Cupping in Hospitalized Covid-19 Patients: A Randomized Open-Label Clinical Trial, Avicenna Journal of Phytomedicine (2023)

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Style	Citing Format
MLA	Salimi A, et al.. "Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme]." Annales Pharmaceutiques Francaises, vol. 83, no. 2, 2025, pp. 311-321.
APA	Salimi A, Hamzeloomoghadam M, Irani M, Esmaeili S (2025). Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme]. Annales Pharmaceutiques Francaises, 83(2), 311-321.
Chicago	Salimi A, Hamzeloomoghadam M, Irani M, Esmaeili S. "Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme]." Annales Pharmaceutiques Francaises 83, no. 2 (2025): 311-321.
Harvard	Salimi A et al. (2025) 'Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme]', Annales Pharmaceutiques Francaises, 83(2), pp. 311-321.
Vancouver	Salimi A, Hamzeloomoghadam M, Irani M, Esmaeili S. Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme]. Annales Pharmaceutiques Francaises. 2025;83(2):311-321.
BibTex	@article{ author = {Salimi A and Hamzeloomoghadam M and Irani M and Esmaeili S}, title = {Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme]}, journal = {Annales Pharmaceutiques Francaises}, volume = {83}, number = {2}, pages = {311-321}, year = {2025} }
RIS	TY - JOUR AU - Salimi A AU - Hamzeloomoghadam M AU - Irani M AU - Esmaeili S TI - Antimalarial Evaluation of Selected Medicinal Plants Traditionally Used for Treatment of Fever, by Inhibition Test of Heme Polymerization; [Evaluation Des Proprietes Antimalariques De Plantes Medicinales Selectionnees Traditionnellement Utilisees Pour Le Traitement De La Fievre Via Le Test D'inhibition De La Polymerisation De L'heme] JO - Annales Pharmaceutiques Francaises VL - 83 IS - 2 SP - 311 EP - 321 PY - 2025 ER -

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