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Selective Immediate Detection of Cu2+ by a Ph-Sensitive Rhodamine-Based Fluorescence Probe in Breast Cancer Cell-Line Publisher Pubmed



Hosseinjanipirdehi H1 ; Allah Mahmoodi NO2 ; Taheri A3 ; Asalemi KAA1 ; Esmaili R4
Authors

Source: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Published:2020


Abstract

A biocompatible fluorogenic chemosensor (N-(3-carboxy acryloyl)) rhodamine B (RHA) comprising a furan-2,5-dione receptor and a rhodamine fluorophore via an iminohydrazine crosslinker has been prepared for more scrutinizing optical properties and utilization in molecular imaging of living cells. The consequences indicated that RHA not only is a good pH indicator in acidic media but also can be used as a great sensitive fluoroionophore with high selectivity. The fluorescence emission of the molecule RHA is quite stable in the presence of 15 diverse cations, 18 anions (especially H2PO4 −, S2, and F−), and 5 amino acids with the different functional group as a model, except Cu2+ ions with observable emission intensity changing. The Cu2+ probe has a dual functional effect, an “on-off” mechanism in solvent media, and an “off-on” mechanism in Phosphate Buffered Saline (PBS) in acidic pH conditions. The detection limits for Cu2+ in acetone-water media was 0.69 μM and 0.18 μM in pH = 5.0. Moreover, RHA can fluorescently and colorimetrically image Cu2+ ions in acidic and neutral pH with low cytotoxicity in living cells. © 2018 Elsevier B.V.
1. Selective Cu2+ Detection by a Novel Fluorescence Hydrazone – Base Probe in Aqueous Media, Journal of Photochemistry and Photobiology A: Chemistry (2021)
3. Environmental Applications of Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
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